The Mucin MUC16 (CA125) Binds to NK Cells and Monocytes from Peripheral Blood of Women with Healthy Pregnancy and Preeclampsia
Chanel Tyler
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorArvinder Kapur
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorMildred Felder
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorJennifer A. Belisle
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorChristine Trautman
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorJennifer A. A. Gubbels
Department of Biology, Augustana College, Sioux Falls, SD, USA
Search for more papers by this authorJoseph P. Connor
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorCorresponding Author
Manish S. Patankar
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Correspondence
Manish S. Patankar, Department of Obstetrics and Gynecology, University of Wisconsin-Madison, H4/657 CSC, 600 Highland Avenue, Madison, WI 53792-6188, USA.
E-mail: [email protected]
Search for more papers by this authorChanel Tyler
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorArvinder Kapur
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorMildred Felder
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorJennifer A. Belisle
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorChristine Trautman
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorJennifer A. A. Gubbels
Department of Biology, Augustana College, Sioux Falls, SD, USA
Search for more papers by this authorJoseph P. Connor
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Search for more papers by this authorCorresponding Author
Manish S. Patankar
Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA
Correspondence
Manish S. Patankar, Department of Obstetrics and Gynecology, University of Wisconsin-Madison, H4/657 CSC, 600 Highland Avenue, Madison, WI 53792-6188, USA.
E-mail: [email protected]
Search for more papers by this authorAbstract
Problem
MUC16 (CA125) released from ovarian tumors binds to NK cells and monocytes via the inhibitory receptor Siglec-9. Here, we investigate whether MUC16 also binds to circulating immune cells during pregnancy and in women with preeclampsia.
Method of study
MUC16 binding was monitored by flow cytometry and immunoprecipitation, and RT-PCR was used to monitor indigenous expression in immune cells. Serum CA125 levels were measured by a clinical assay.
Results
MUC16 was equally distributed on Siglec-9pos CD16pos/CD56dim and CD16neg/CD56br NK cells in the healthy pregnant and preeclampsia groups. While serum CA125 levels and number of NK and monocytes were similar, increased binding of MUC16 was observed on these immune cells in the preeclampsia cohort as compared to the healthy pregnant samples.
Conclusion
MUC16 binding to NK cells and monocytes likely contributes to tolerance of the fetal allograft from maternal responses and may also serve as a novel biomarker for preeclampsia.
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