TGFβ2 in Rabbit Blastocoelic Fluid Regulates CD4 Membrane Expression: Possible Role in the Success of Gestation
Marie-Josée Ouellette
Departments of Obstetrics and Gynecology, Laval University, and Ontogénie et Reproduction-CRBR, C.H.U.L. Research Center, Ste-Foy, Québec, Canada
Search for more papers by this authorClaire M. Dubois
Laboratoire d'immunologie moléculaire et cellulaire, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada
Search for more papers by this authorDominic Bergeron
Departments of Obstetrics and Gynecology, Laval University, and Ontogénie et Reproduction-CRBR, C.H.U.L. Research Center, Ste-Foy, Québec, Canada
National Research Council of Canada, Institute for Biological Sciences, Building M-54, Ottawa, Ontario, Canada
Search for more papers by this authorRaynald Roy
Centre de recherche en rhumatologie et immunologie, C.H.U.L., Ste-Foy, Québec, Canada
Search for more papers by this authorCorresponding Author
Dr. Raymond D. Lambert
Departments of Obstetrics and Gynecology, Laval University, and Ontogénie et Reproduction-CRBR, C.H.U.L. Research Center, Ste-Foy, Québec, Canada
Unité de recherche en Ontogénie et reproduction, Centre de recherche du C.H.U.L., 2705 Boulevard Laurier, Ste-Foy, Québec, Canada, G1V-4G2.Search for more papers by this authorMarie-Josée Ouellette
Departments of Obstetrics and Gynecology, Laval University, and Ontogénie et Reproduction-CRBR, C.H.U.L. Research Center, Ste-Foy, Québec, Canada
Search for more papers by this authorClaire M. Dubois
Laboratoire d'immunologie moléculaire et cellulaire, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada
Search for more papers by this authorDominic Bergeron
Departments of Obstetrics and Gynecology, Laval University, and Ontogénie et Reproduction-CRBR, C.H.U.L. Research Center, Ste-Foy, Québec, Canada
National Research Council of Canada, Institute for Biological Sciences, Building M-54, Ottawa, Ontario, Canada
Search for more papers by this authorRaynald Roy
Centre de recherche en rhumatologie et immunologie, C.H.U.L., Ste-Foy, Québec, Canada
Search for more papers by this authorCorresponding Author
Dr. Raymond D. Lambert
Departments of Obstetrics and Gynecology, Laval University, and Ontogénie et Reproduction-CRBR, C.H.U.L. Research Center, Ste-Foy, Québec, Canada
Unité de recherche en Ontogénie et reproduction, Centre de recherche du C.H.U.L., 2705 Boulevard Laurier, Ste-Foy, Québec, Canada, G1V-4G2.Search for more papers by this authorAbstract
PROBLEM: During pregnancy, major changes occur in the decidual cell population. One of these changes involves some phenotypical transformations of lymphocyte sub-populations. Since these variations might be due to the presence of the embryo, the current study was designed to investigate the implication of blastocoelic fluid (BF) in these changes and to determine the mechanism by which this phenomenon occurs.
METHOD: Lymphocytes isolated from human peripheral blood (PBL) were cultured for 72 h in RPMI-FCS 10% and with or without BF day 12 (BF d-12) or Concanavalin A (ConA). After 72 h, T cells were labelled with anti-CD4 antibodies and Quantum Simply Cellular microbeads were used as a standard to evaluate the antibody binding capacity (ABC).
RESULTS: Treatment of human PBL with BF d-12 decreases the percentage of CD4 and TCR positive cells, as compared to non-stimulated cells, but has no significant effect on CD2, CD3, and CD8 positive cells. It was also demonstrated, for the first time, that transforming growth factor beta-2 (TGFβ2) in BF day 12 diminishes the percentage of CD4 positive cells by downregulating CD4 membrane expression on leucocytes.
CONCLUSION: These findings suggest that the embryo plays a key role in its own protection. Furthermore, it is predicted that any tissue producing TGFβ2, such as certain types of tumor, downregulates the immune response, thus allowing tumor growth.
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