Photodermatology, Photoimmunology & Photomedicine

A study of Q-switched Nd:YAG laser irradiation and paracrine function in human skin cells

Andrew Burd

Division of Plastic and Reconstructive Surgery, Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong

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Ningwen Zhu,

Ningwen Zhu

Department of Reconstructive Burns & Plastic Surgery, Northern General Hospital Trust, Sheffield, UK

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Vincent K. M. Poon,

Vincent K. M. Poon

Division of Plastic and Reconstructive Surgery, Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong

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Andrew Burd,

Andrew Burd

Division of Plastic and Reconstructive Surgery, Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong

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Ningwen Zhu,

Ningwen Zhu

Department of Reconstructive Burns & Plastic Surgery, Northern General Hospital Trust, Sheffield, UK

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Vincent K. M. Poon,

Vincent K. M. Poon

Division of Plastic and Reconstructive Surgery, Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong

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First published: 11 May 2005

https://doi.org/10.1111/j.1600-0781.2005.00155.x

Citations: 22

Corresponding author:
Professor Andrew Burd
Chief of Division of Plastic & Reconstructive Surgery
Department of Surgery
Prince of Wales Hospital, Shatin
Hong Kong
Tel: 852-2632-2639
Fax: 852-2632-4675
e-mail: [email protected]

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Abstract

Background and objectives: This preliminary laboratory-based study looks at the paracrine release from human skin cells subject to sublethal Q-switched Nd:YAG 532 nm laser irradiation.

Study design/Materials and methods: Human dermal fibroblast and keratinocyte cultures were exposed to sublethal energy using the Nd:YAG 532 nm laser. Altered gene expression was then screened using RT-PCR for a range of paracrine factors known to affect melanogenesis, basic fibroblast growth factor (b-FGF), hepatocyte growth factor (HGF), stem cell factor (SCF), melanocyte stimulating hormone (MSH), endothelin-1 (ET-1), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and protease-activated receptor-2 (PAR-2). Enzyme-linked immunosorbent assay (ELISA) was used to confirm protein production. Conditioned medium was used to assess altered melanogenesis in a melanoma cell line.

Results: Fibroblasts exposed to sublethal radiation showed upregulation of b-FGF, HGF and SCF. This contrasts with keratinocytes which showed upregulation of IL-6. Elevated protein levels of b-FGF and SCF were confirmed by ELISA assay. Conditioned fibroblast medium was shown to stimulate melanogenesis in a melanoma cell line.

Conclusions: This preliminary laboratory study reports, for the first time, specific gene upregulation using the Q-switched Nd:YAG 532 nm laser.

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Volume21, Issue3

June 2005

Pages 131-137

A study of Q-switched Nd:YAG laser irradiation and paracrine function in human skin cells

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A study of Q-switched Nd:YAG laser irradiation and paracrine function in human skin cells

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