Phospholipase C-γ1 expression correlated with cancer progression of potentially malignant oral lesions

Li-Wei Ma

Shanghai Key Laboratory of Stomatology, Department of Oral Mucosal Diseases, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

Zeng-Tong Zhou,

Zeng-Tong Zhou

Shanghai Key Laboratory of Stomatology, Department of Oral Mucosal Diseases, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

Qing-Bo He,

Qing-Bo He

Department of Bio-statistics, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

Wei-Wen Jiang,

Wei-Wen Jiang

Shanghai Key Laboratory of Stomatology, Department of Oral Mucosal Diseases, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

Li-Wei Ma,

Li-Wei Ma

Shanghai Key Laboratory of Stomatology, Department of Oral Mucosal Diseases, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

Zeng-Tong Zhou,

Zeng-Tong Zhou

Shanghai Key Laboratory of Stomatology, Department of Oral Mucosal Diseases, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

Qing-Bo He,

Qing-Bo He

Department of Bio-statistics, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

Wei-Wen Jiang,

Wei-Wen Jiang

Shanghai Key Laboratory of Stomatology, Department of Oral Mucosal Diseases, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Search for more papers by this author

First published: 05 June 2012

https://doi.org/10.1111/j.1600-0714.2012.01179.x

Citations: 14

Wei-Wen Jiang, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China. Tel: +86 21 23271699, Fax: +86 21 63087076, E-mail: [email protected]

Share a link

Email
Wechat
Bluesky

Abstract

Background: Phospholipase C-γ1 (PLCγ1) is required for cellular migration during tumor progression and invasion of oral squamous cell carcinoma (OSCC) cells. The objective of the current study was to determine immunoexpression pattern of PLCγ1 in oral potentially malignant lesions (OPLs) and evaluate PLCγ1 usefulness as a biomarker for predicting clinical behavior in the carcinogenesis of OPL.

Methods: In a retrospective follow-up study, the expression pattern of PLCγ1 protein was determined using immunohistochemistry in samples from 68 patients, including untransformed cases (n = 38) and malignant-transformed cases (n = 30). The corresponding post-malignant lesions (OSCCs) were also performed.

Results: We observed that elevated expression of PLCγ1 in 40 of 68 (59%) general OPLs and 23 of 30 (77%) OSCCs compared with that in normal oral mucosa. Kaplan–Meier analysis revealed that patients with PLCγ1 positivity had a significantly higher incidence of OSCC than those with PLCγ1 negativity. Cox regression analysis revealed that PLCγ1 expression patterns were significantly associated with increased risk of malignant progression. In addition, the correlation between PLCγ1 expression in pre-malignant OPL and that in post-malignant OSCC was significant (P = 0.004).

Conclusion: These data indicate that PLCγ1 expression in OPL correlated with oral cancer progression, and PLCγ1 may serve as a useful marker for the identification of high-risk OPL into OSCC.

Supporting Information

References

1Warnakulasuriya S. Living with oral cancer: epidemiology with particular reference to prevalence and life-style changes that influence survival. Oral Oncol2010; 46: 407–10.
10.1016/j.oraloncology.2010.02.015
PubMed Web of Science® Google Scholar
2Zini A, Czerninski R, Sgan-Cohen HD. Oral cancer over four decades: epidemiology, trends, histology, and survival by anatomical sites. J Oral Pathol Med2010; 39: 299–305.
10.1111/j.1600-0714.2009.00845.x
CAS PubMed Web of Science® Google Scholar
3Ganly I, Patel S, Shah J. Early stage squamous cell cancer of the oral tongue- clinicopathologic features affecting outcome. Cancer2011; 118: 101–11.
10.1002/cncr.26229
PubMed Web of Science® Google Scholar
4van der Waal I. Potentially malignant disorders of the oral and oropharyngeal mucosa; present concepts of management. Oral Oncol2010; 46: 423–5.
10.1016/j.oraloncology.2010.02.016
PubMed Web of Science® Google Scholar
5Pitiyage G, Tilakaratne WM, Tavassoli M, Warnakulasuriya S. Molecular markers in oral epithelial dysplasia: review. J Oral Pathol Med2009; 38: 737–52.
10.1111/j.1600-0714.2009.00804.x
CAS PubMed Web of Science® Google Scholar
6Smith J, Rattay T, McConkey C, Helliwell T, Mehanna H. Biomarkers in dysplasia of the oral cavity: a systematic review. Oral Oncol2009; 45: 647–53.
10.1016/j.oraloncology.2009.02.006
CAS PubMed Web of Science® Google Scholar
7Mehanna HM, Rattay T, Smith J, McConkey CC. Treatment and follow-up of oral dysplasia - a systematic review and meta-analysis. Head Neck2009; 31: 1600–9.
10.1002/hed.21131
PubMed Web of Science® Google Scholar
8Nankivell P, Mehanna H. Oral Dysplasia: Biomarkers, Treatment, and Follow-up. Curr Oncol Rep2011; 13: 145–52.
10.1007/s11912-010-0150-z
PubMed Web of Science® Google Scholar
9Lingen MW, Pinto A, Mendes RA, et al.Genetics/epigenetics of oral premalignancy: current status and future research. Oral Dis2011; 17(Suppl. 1): 7–22.
10.1111/j.1601-0825.2011.01789.x
PubMed Web of Science® Google Scholar
10Wu RQ, Zhao XF, Wang ZY, Zhou M, Chen QM. Novel molecular events in oral carcinogenesis via integrative approaches. J Dent Res2011; 90: 561–72.
10.1177/0022034510383691
CAS PubMed Web of Science® Google Scholar
11Wells A, Grandis JR. Phospholipase C-γ1 in tumor progression. Clin Exp Metastasis2003; 20: 285–90.
10.1023/A:1024088922957
CAS PubMed Web of Science® Google Scholar
12Sala G, Dituri F, Raimondi C, et al.Phospholipase Cγ1 is required for metastasis development and progression. Cancer Res2008; 68: 10187–96.
10.1158/0008-5472.CAN-08-1181
CAS PubMed Web of Science® Google Scholar
13Szabó B, Nelhubel GA, Kárpáti A, et al.Clinical significance of genetic alterations and expression of epidermal growth factor receptor (EGFR) in head and neck squamous cell carcinomas. Oral Oncol2011; 47: 487–96.
10.1016/j.oraloncology.2011.03.020
CAS PubMed Web of Science® Google Scholar
14Sheu JJ, Hua CH, Wan L, et al.Functional genomic analysis identified epidermal growth factor receptor activation as the most common genetic event in oral squamous cell carcinoma. Cancer Res2009; 69: 2568–76.
10.1158/0008-5472.CAN-08-3199
CAS PubMed Web of Science® Google Scholar
15Wu HG, Song SY, Kim YS, et al.Therapeutic effect of recombinant human epidermal growth factor (RhEGF) on mucositis in patients undergoing radiotherapy, with or without chemotherapy, for head and neck cancer: a double-blind placebo-controlled prospective phase 2 multi-institutional clinical trial. Cancer2009; 115: 3699–708.
10.1002/cncr.24414
CAS PubMed Web of Science® Google Scholar
16Sharafinski ME, Ferris RL, Ferrone S, Grandis JR. Epidermal growth factor receptor targeted therapy of squamous cell carcinoma of the head and neck. Head Neck2010; 32: 1412–21.
10.1002/hed.21365
PubMed Web of Science® Google Scholar
17Taoudi Benchekroun M, Saintigny P, Thomas SM, et al.Epidermal growth factor receptor expression and gene copy number in the risk of oral cancer. Cancer Prev Res2010; 3: 800–9.
10.1158/1940-6207.CAPR-09-0163
CAS PubMed Web of Science® Google Scholar
18Shepard CR, Kassis J, Whaley DL, Kim HG, Wells A. PLCγ contributes to metastasis of in situ-occurring mammary and prostate tumors. Oncogene2007; 26: 3020–6.
10.1038/sj.onc.1210115
CAS PubMed Web of Science® Google Scholar
19Kang JK, Chang CH, Nam HJ, et al.Downregulation of erythropoietin receptor by overexpression of phospholipase C-gamma 1 is critical for decrease on focal adhesion in transformed cells. Cell Oncol2011; 34: 11–21.
10.1007/s13402-010-0001-9
CAS Web of Science® Google Scholar
20Xie Z, Chen Y, Liao EY, Jiang Y, Liu FY, Pennypacker SD. Phospholipase C-gamma1 is required for the epidermal growth factor receptor-induced squamous cell carcinoma cell mitogenesis. Biochem Biophys Res Commun2010; 397: 296–300.
10.1016/j.bbrc.2010.05.103
CAS PubMed Web of Science® Google Scholar
21Nozawa H, Howell G, Suzuki S, et al.Combined inhibition of PLCγ-1 and c-Src abrogates epidermal growth factor receptor-mediated head and neck squamous cell carcinoma invasion. Clin Cancer Res2008; 14: 4336–44.
10.1158/1078-0432.CCR-07-4857
CAS PubMed Web of Science® Google Scholar
22Thomas SM, Coppelli FM, Wells A, et al.Epidermal growth factor receptor-stimulated activation of phospholipase C -1 promotes invasion of head and neck squamous cell carcinoma. Cancer Res2003; 63: 5629–35.
CAS PubMed Web of Science® Google Scholar
23Beloueche-Babari M, Peak JC, Jackson LE, Tiet MY, Leach MO, Eccles SA. Changes in choline metabolism as potential biomarkers of phospholipase C{gamma}1 inhibition in human prostate cancer cells. Mol Cancer Ther2009; 8: 1305–11.
10.1158/1535-7163.MCT-09-0039
CAS PubMed Web of Science® Google Scholar
24Torres-Rendon A, Roy S, Craig GT, Speight PM. Expression of Mcm2, geminin and Ki67 in normal oral mucosa, oral epithelial dysplasias and their corresponding squamous-cell carcinomas. Br J Cancer2009; 100: 1128–34.
10.1038/sj.bjc.6604967
CAS PubMed Web of Science® Google Scholar
25Montebugnoli L, Cervellati F, Cocchi R, et al.Immunohistochemical expression of p16(INK4A) protein as a helpful marker of a subset of potentially malignant oral epithelial lesions: study on a series with long-term follow-up. Histopathology2010; 57: 528–34.
10.1111/j.1365-2559.2010.03671.x
CAS PubMed Web of Science® Google Scholar
26Liu W, Wang YF, Zhou HW, Shi P, Zhou ZT, Tang GY. Malignant transformation of oral leukoplakia: a retrospective cohort study of 218 Chinese patients. BMC Cancer2010; 10: 685.
10.1186/1471-2407-10-685
PubMed Web of Science® Google Scholar
27Ogbureke KU, Abdelsayed RA, Kushner H, Li L, Fisher LW. Two members of the SIBLING family of proteins, DSPP and BSP, may predict the transition of oral epithelial dysplasia to oral squamous cell carcinoma. Cancer2010; 116: 1709–17.
10.1002/cncr.24938
PubMed Web of Science® Google Scholar
28Liu W, Feng JQ, Shen XM, Wang HY, Liu Y, Zhou ZT. Two stem cell markers, ATP-binding cassette, G2 subfamily (ABCG2) and BMI-1, predict the transformation of oral leukoplakia to cancer: a long-term follow-up study. Cancer2012; 118: 1693–700.
10.1002/cncr.26483
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume42, Issue1

January 2013

Pages 47-52

Phospholipase C-γ1 expression correlated with cancer progression of potentially malignant oral lesions

Abstract

Supporting Information

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Phospholipase C-γ1 expression correlated with cancer progression of potentially malignant oral lesions

Abstract

Supporting Information

References

Citing Literature

References

Related

Information