Volume 119, Issue 7 pp. 468-478

Sublingual vaccination with sonicated Salmonella proteins and mucosal adjuvant induces mucosal and systemic immunity and protects mice from lethal enteritis

CHING-FENG HUANG

CHING-FENG HUANG

Department of Pediatrics, Tri-Service General Hospital, National Defense Medical Center, Taipei

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TZEE-CHUNG WU

TZEE-CHUNG WU

Department of Pediatrics, Taipei Veterans General Hospital, Taipei

School of Medicine, National Yang-Ming University, Taipei

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CHIA-CHAO WU

CHIA-CHAO WU

Division of Nephrology, Department of Medicine, Tri-Service General Hospital, Taipei

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CHIN-CHENG LEE

CHIN-CHENG LEE

Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei

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WEN-TSUNG LO

WEN-TSUNG LO

Department of Pediatrics, Tri-Service General Hospital, National Defense Medical Center, Taipei

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KWEI-SHUAI HWANG

KWEI-SHUAI HWANG

Department of Obstetrics and Gynecology, Tri-Service General Hospital, Taipei

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MU-LING HSU

MU-LING HSU

Department of Pediatrics, Hualien Armed Forces General Hospital, Hualien

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HO-JEN PENG

HO-JEN PENG

Department of Pediatrics, Taipei Veterans General Hospital, Taipei

Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan

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First published: 14 May 2011
Citations: 10
Ho-Jen Peng, Department of Medical Research and Education, Taipei Veterans General Hospital, No. 201, Sec. 2, Shih-Pai Road, Taipei 11217, Taiwan. e-mail: [email protected]

Abstract

Huang C-F, Wu T-C, Wu C-C, Lee C-C, Lo W-T, Hwang K-S, Hsu M-L, Peng H-J. Sublingual vaccination with sonicated Salmonella proteins and mucosal adjuvant induces mucosal and systemic immunity and protects mice from lethal enteritis. APMIS 2011; 119: 468–78.

Salmonella enteritidis is one of the most common pathogens of enteritis. Most experimental vaccines against Salmonella infection have been applied through injections. This is a new trial to explore the effect of sublingual administration of Salmonella vaccines on systemic and mucosal immunity. Adult BALB/c mice were sublingually vaccinated with sonicated Salmonella proteins (SSP) alone, or plus adjuvant CpG DNA (CpG) or cholera toxin (CT). They were boosted 2 weeks later. Saliva specific secretory IgA (SIgA) antibody responses were significantly stimulated in the mice vaccinated with SSP only or together with CpG or CT. Whereas the mice sublingually vaccinated with SSP and CpG had higher spleen cell IFN-γ production and serum specific IgG2a antibody responses, those receiving SSP and CT showed enhanced spleen cell IL-4, IL-5 and IL-6 production, and serum specific IgG1 antibody responses. After oral challenge with live S. enteritidis, the same strain of the source of SSP, immune protection in those sublingually vaccinated with SSP and CpG or CT was found to prevent intestinal necrosis and to render a higher survival rate. In conclusion, sublingual vaccination together with mucosal adjuvant CpG or CT is a simple but effective way against enteric bacterial pathogens.

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