SipA, a novel type of protein from Synechococcus sp. PCC 7942, binds to the kinase domain of NblS
Javier Espinosa,
Inmaculada Fuentes,
Sergio Burillo,
Francisco Rodríguez-Mateos,
Asunción Contreras,
Javier Espinosa
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorInmaculada Fuentes
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorSergio Burillo
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorFrancisco Rodríguez-Mateos
Departamento de Matemática Aplicada, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorAsunción Contreras
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorJavier Espinosa,
Inmaculada Fuentes,
Sergio Burillo,
Francisco Rodríguez-Mateos,
Asunción Contreras,
Javier Espinosa
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorInmaculada Fuentes
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorSergio Burillo
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorFrancisco Rodríguez-Mateos
Departamento de Matemática Aplicada, Universidad de Alicante, Alicante, Spain
Search for more papers by this authorAsunción Contreras
División de Genética, Universidad de Alicante, Alicante, Spain
Search for more papers by this author Asunción Contreras, División de Genética, Facultad de Ciencias, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain. Tel.: +34 96 590 3957; fax: +34 96 590 9569; e-mail: [email protected]
Editor: Karl Forchhammer
Abstract
Cyanobacteria respond to nutrient stress conditions by degrading their light-harvesting complexes for photosynthesis, a process regulated in Synechococcus sp. PCC 7942 by the sensor histidine kinase non-bleaching sensor (NblS). In yeast two-hybrid screenings for proteins interacting with NblS we have identified a novel type of protein, named SipA for NblS interacting protein A. Specific binding between NblS and SipA is observed with both yeast and bacterial two-hybrid systems. Additional yeast two-hybrid screenings with SipA as bait further confirmed the specificity of the interaction and allowed us to map their determinants to the ATP-binding domain of NblS. Strong conservation and coevolution of both NblS and SipA in cyanobacteria further suggests the importance of SipA in the context of the NblS signal transduction network.
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