Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction
Corresponding Author
Bernard China
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
*Corresponding author. Tel.: +32 (4) 366 40 52; Fax: +32 (4) 366 40 55, E-mail address: [email protected]Search for more papers by this authorFrédéric Goffaux
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
Search for more papers by this authorVinciane Pirson
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
Search for more papers by this authorJacques Mainil
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
Search for more papers by this authorCorresponding Author
Bernard China
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
*Corresponding author. Tel.: +32 (4) 366 40 52; Fax: +32 (4) 366 40 55, E-mail address: [email protected]Search for more papers by this authorFrédéric Goffaux
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
Search for more papers by this authorVinciane Pirson
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
Search for more papers by this authorJacques Mainil
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43a, B-4000 Liège, Belgium
Search for more papers by this authorAbstract
Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype.
References
- [1] Mainil, J.G., Jacquemin, E., Kaeckenbeeck, A., Pohl, P. (1993) Association between the effacing gene (eae) and the Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle. Am. J. Vet. Res. 54, 1064–1068.
- [2] China, B., Pirson, V., Mainil, J. (1998) Prevalence and molecular typing of attaching and effacing Escherichia coli among calf populations in Belgium. Vet. Microbiol. 63, 249–259.
- [3] Nataro, J.P., Kaper, J.B. (1998) Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11, 142–201.
- [4] McDaniel, T.K., Jarvis, G., Donnenberg, M.S., Kaper, J.B. (1995) A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl. Acad. Sci. USA 92, 1664–1668.
- [5] McDaniel, T.K., Kaper, J.B. (1997) A cloned pathogenesis island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K12. Mol. Microbiol. 23, 399–407.
- [6] Elliott, S.J., Wainwright, L.A., McDaniel, T.K., Jarvis, K.G., Deng, Y.K., Lai, L.C., McNamara, B.P., Donnenberg, M.S., Kaper, J.B. (1998) The complete sequence of the locus of enteroycte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol. Microbiol. 28, 1–4.
- [7] Perna, N., Mayhew, G.F., Posfai, G., Elliot, S., Donnenberg, M.S., Kaper, J.B., Blattner, F.R. (1998) Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66, 3810–3817.
- [8] Karaolis, D.K.R., McDaniel, T.K., Kaper, J.B. and Boedeker, E.C. (1997) Cloning of the RDEC-1 locus of enterocyte effacement (LEE) and functional analysis of the phenotype on Hep-2 cells. In: Mechanisms in the Pathogenesis of Enteric Diseases (Paul, P.S., Francis, D.H. and Benfield, D.A., Eds.), Adv. Exp. Med. Biol. 412, pp. 241–245. Plenum, New York.
- [9] Goffaux, F., Mainil, J., Pirson, V., Charlier, G., Pohl, P., Jacquemin, E., China, B. (1997) Bovine attaching and effacing Escherichia coli possess a pathogenesis island related to the LEE of the human enteropathogenic E. coli strain E2348/69. FEMS Microbiol. Lett. 154, 415–421.
- [10] Agin, T.S., Cantey, J.R., Boedeker, E.C., Wolf, M.K. (1996) Characterization of the eaeA gene from rabbit enteropathogenic Escherichia coli RDEC-1 and comparison to other eaeA genes from bacteria that cause attaching-afacing lesions. FEMS Microbiol. Lett. 14, 249–258.
- [11] Abe, A., Kenny, B., Stein, M., Finlay, B.B. (1997) Characterization of two virulence proteins secreted by rabbit enteropathogenic Escherichia coli, EspA and EspB, whose maximal expression is sensitive to host body temperature. Infect. Immun. 65, 3547–3555.
- [12] Paton, A.W., Mannig, P.A., Woodrow, M.C., Paton, J.C. (1998) Translocated intimin receptor (Tir) of Shiga-toxigenic Escherichia coli isolates belonging to serogroups, O26, O111, and O157 react with sera from patients with hemolytic-Uremic syndrome and exhibit marked sequence heterogeneity. Infect. Immun. 66, 5580–5586.
- [13] An, H., Fairbrother, J.M., Dubreuil, D., Harel, J. (1997) Cloning and characterization of the eae gene from a dog attaching and effacing Escherichia coli strain 4221. FEMS Microbiol. Lett. 148, 239–245.
- [14] China, B., Devrin, A.C., Jacquemin, E., Pirson, V., Mainil, J. (1999) Heterogeneity of the eae genes in attaching/effacing Escherichia coli from cattle: comparison with human strains. Res. Microbiol. 150, 323–332.
- [15] Adu-Bobie, J., Frankel, G., Bain, C., Gonzales, A.G., Trabulsi, L.R., Douce, G., Knutton, S., Dougan, G. (1998) Detection of intimins α, β, γ, and δ four intimin derivates expressed by attaching and effacing microbial pathogens. J. Clin. Microbiol. 36, 662–668.
- [16] Jerse, A.E., Yu, J., Tall, B.D., Kaper, J.B. (1990) A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc. Natl. Acad. Sci. USA 87, 7839–7843.
- [17] Orskov, F., Orskov, I. (1984) Serotyping of Escherichia coli. Method. Microbiol. 14, 43–112.
- [18] China, B., Pirson, V., Mainil, J. (1996) Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl. Environ. Microbiol. 62, 3462–3465.
- [19] Blanco, M., Blanco, J.E., Blanco, J., Moro, A., Prado, C., Alonso, M.P., Mourino, M., Madrid, C., Balsalobre, C., Juarez, A. (1997) Distribution and characterization of faecal verotoxin-producing Escherichia coli (VTEC) isolated from healthy cattle. Vet. Microbiol. 54, 309–319.
