Increased thermosensitivity associated with topoisomerase I deletion and promoter mutations in Escherichia coli
Haiyan Qi
Department of Biochemistry and Molecular Biology, Basic Science Building, Room 128, New York Medical College, Valhalla, NY 10595, USA
Search for more papers by this authorRolf Menzel
Small Molecule Therapeutics, 11 Deer Park Drive, Suite 116, Monmouth Junction, NJ 08852, USA
Search for more papers by this authorCorresponding Author
Yuk-Ching Tse-Dinh
Department of Biochemistry and Molecular Biology, Basic Science Building, Room 128, New York Medical College, Valhalla, NY 10595, USA
*Corresponding author. Tel.: +1 (914) 594-4061; Fax: +1 (914) 594-4058, E-mail address: [email protected]Search for more papers by this authorHaiyan Qi
Department of Biochemistry and Molecular Biology, Basic Science Building, Room 128, New York Medical College, Valhalla, NY 10595, USA
Search for more papers by this authorRolf Menzel
Small Molecule Therapeutics, 11 Deer Park Drive, Suite 116, Monmouth Junction, NJ 08852, USA
Search for more papers by this authorCorresponding Author
Yuk-Ching Tse-Dinh
Department of Biochemistry and Molecular Biology, Basic Science Building, Room 128, New York Medical College, Valhalla, NY 10595, USA
*Corresponding author. Tel.: +1 (914) 594-4061; Fax: +1 (914) 594-4058, E-mail address: [email protected]Search for more papers by this authorAbstract
An Escherichia coli mutant with three of the promoters for the topoisomerase I gene (topA) deleted, such that only the σ32-dependent promoter (P1) remained, had a decreased level of topoisomerase I at 30°C and showed increased thermosensitivity at 52°C. However, it could still develop thermotolerance and had a wild-type level of resistance to 52°C treatment if exposed first to 42°C. This indicated that newly synthesized topoisomerase I from transcription initiated at P1 was important for development of thermotolerance. Two other E. coli mutants lacking topA were >100 times more sensitive to high temperature than their wild-type isogenic strains.
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