Isolation and characterization of a thermophilic Bacillus sp. JF8 capable of degrading polychlorinated biphenyls and naphthalene
Corresponding Author
Minoru Shimura
Environmental Biotechnology Laboratory, Railway Technical Research Institute, 2-8-38 Hikari-cho, Kokubunji-shi, Tokyo 185-8540, Japan
*Corresponding author. Tel.: +81 (42) 573-7316; Fax: +81 (42) 573-7349, E-mail address: [email protected]Search for more papers by this authorGouri Mukerjee-Dhar
Environmental Biotechnology Laboratory, Railway Technical Research Institute, 2-8-38 Hikari-cho, Kokubunji-shi, Tokyo 185-8540, Japan
Search for more papers by this authorKazuhide Kimbara
Environmental Biotechnology Laboratory, Railway Technical Research Institute, 2-8-38 Hikari-cho, Kokubunji-shi, Tokyo 185-8540, Japan
Search for more papers by this authorHiroko Nagato
Research Institute of Technology, Okayama Univ. of Science, 401-1 Seki, Okayama-shi, Okayama 703-8232, Japan
Search for more papers by this authorHohzoh Kiyohara
Research Institute of Technology, Okayama Univ. of Science, 401-1 Seki, Okayama-shi, Okayama 703-8232, Japan
Search for more papers by this authorTakashi Hatta
Research Institute of Technology, Okayama Univ. of Science, 401-1 Seki, Okayama-shi, Okayama 703-8232, Japan
Search for more papers by this authorCorresponding Author
Minoru Shimura
Environmental Biotechnology Laboratory, Railway Technical Research Institute, 2-8-38 Hikari-cho, Kokubunji-shi, Tokyo 185-8540, Japan
*Corresponding author. Tel.: +81 (42) 573-7316; Fax: +81 (42) 573-7349, E-mail address: [email protected]Search for more papers by this authorGouri Mukerjee-Dhar
Environmental Biotechnology Laboratory, Railway Technical Research Institute, 2-8-38 Hikari-cho, Kokubunji-shi, Tokyo 185-8540, Japan
Search for more papers by this authorKazuhide Kimbara
Environmental Biotechnology Laboratory, Railway Technical Research Institute, 2-8-38 Hikari-cho, Kokubunji-shi, Tokyo 185-8540, Japan
Search for more papers by this authorHiroko Nagato
Research Institute of Technology, Okayama Univ. of Science, 401-1 Seki, Okayama-shi, Okayama 703-8232, Japan
Search for more papers by this authorHohzoh Kiyohara
Research Institute of Technology, Okayama Univ. of Science, 401-1 Seki, Okayama-shi, Okayama 703-8232, Japan
Search for more papers by this authorTakashi Hatta
Research Institute of Technology, Okayama Univ. of Science, 401-1 Seki, Okayama-shi, Okayama 703-8232, Japan
Search for more papers by this authorAbstract
Bacillus sp. strain JF8, which was isolated from compost, utilizes naphthalene and biphenyl as carbon sources at 60°C. Biphenyl grown cells of strain JF8 barely degraded naphthalene while naphthalene grown cells did not degrade p-chlorobiphenyl, suggesting the existence of two independent degradation pathways. Isolation of JF8N, a mutant strain which can not utilize biphenyl as a carbon source while retaining the ability to utilize naphthalene, supports this hypothesis. Biphenyl grown cells of strain JF8 can degrade several polychlorinated biphenyl congeners including tetra- and pentachlorobiphenyl. bph and nah probes from mesophilic organisms failed to hybridize to strain JF8 DNA.
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