Transcriptional analysis of the cyclophilin A gene (cypA) of Streptomyces chrysomallus

Rico Berger

Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstrasse 29, D-10587 Berlin-Charlottenburg, Germany

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Florian Schauwecker,

Florian Schauwecker

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Ullrich Keller,

Corresponding Author

Ullrich Keller

*Corresponding author. Tel.: +49 (30) 314 25653; Fax: +49 (30) 314 73522, E-mail address: [email protected]Search for more papers by this author

Rico Berger,

Rico Berger

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Florian Schauwecker,

Florian Schauwecker

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Ullrich Keller,

Corresponding Author

Ullrich Keller

*Corresponding author. Tel.: +49 (30) 314 25653; Fax: +49 (30) 314 73522, E-mail address: [email protected]Search for more papers by this author

First published: 17 January 2006

https://doi.org/10.1111/j.1574-6968.1999.tb13757.x

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Abstract

Streptomyces chrysomallus produces a 17.5-kDa cyclophilin (CypA) as a major cytosolic protein. Northern blotting of total RNA from S. chrysomallus showed that cypA is transcribed as a monocistronic 500-nt transcript during the vegetative growth phase only. Consistently, Western blot analysis and enzyme activity determinations revealed a high level of CypA which declined drastically when cultures entered postexponential phase. Primer extension experiments revealed that cypA is transcribed as a leaderless transcript with the startpoint of transcription and translation being the same AUG codon. Analysis of −10 and −35 regions revealed an Eσ^hrdB-specific promoter consensus sequence in accordance with the observed transcription during vegetative growth of cultures. The leaderless cypA mRNA sequence between codons 5 to 12 shows complementarity to an internal antidownstream box of 16S rRNA of Streptomyces lividans. This may indicate a possible interaction of the leaderless cypA transcript with the 16S mRNA in translation initiation.

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Volume178, Issue1

September 1999

Pages 39-45

Transcriptional analysis of the cyclophilin A gene (cypA) of Streptomyces chrysomallus

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Transcriptional analysis of the cyclophilin A gene (cypA) of Streptomyces chrysomallus

Abstract

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