Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transformed to the acu⁺ phenotype with a clone of ACS (facA) from Aspergillus nidulans. The acuB mutant was transformed with the A. niger facB clone which has been identified by cross-hybridisation of an A. nidulans facB clone. These results confirm that acuA in A. niger is the gene for ACS and acuB is analogous to the A. nidulans facB regulatory gene.

References

[1] Sealy-Lewis, H.M., Fairhurst, V. (1998) Isolation of mutants deficient in acetyl-CoA synthetase and a possible regulator of acetate induction in Aspergillus niger. Microbiology 144, 1895–1900.
10.1099/00221287-144-7-1895
CAS PubMed Web of Science® Google Scholar
[2] Apirion, D. (1965) The two way selection of mutants and revertants in respect of acetate utilisation and resistance to fluoroacetate in Aspergillus nidulans. Genet. Res. 6, 317–329.
10.1017/S0016672300004213
CAS PubMed Web of Science® Google Scholar
[3] Armitt, S., McCullough, W., Roberts, C.R. (1976) Analysis of acetate non-utilising (acu) mutants in Aspergillus nidulans. J. Gen. Microbiol. 92, 263–282.
10.1099/00221287-92-2-263
CAS PubMed Web of Science® Google Scholar
[4] Sealy-Lewis, H.M. (1994) A new selection method for isolating mutants defective in acetate utilisation in Aspergillus nidulans. Curr. Genet. 25, 47–48.
10.1007/BF00712966
CAS PubMed Web of Science® Google Scholar
[5] Sandeman, R.A., Hynes, M.J. (1989) Isolation of the facA (acetyl-Coenzyme A synthetase) and acuE (malate synthase) genes of Aspergillus nidulans. Mol. Gen. Genet. 218, 87–92.
10.1007/BF00330569
CAS PubMed Web of Science® Google Scholar
[6] Katz, M.E., Hynes, M.J. (1989) Isolation and analysis of the acetate regulatory gene, facB, from Aspergillus nidulans. Mol. Cell. Biol. 9, 5696–5701.
10.1128/MCB.9.12.5696
CAS PubMed Web of Science® Google Scholar
[7] Todd, R.B., Murphy, R.L., Martin, H.M., Sharp, J.A., Davis, M., Katz, M.E., Hynes, M.J. (1997) The acetate regulatory gene facB of Aspergillus nidulans encodes a Zn(II)2Cys6 transcriptional activator. Mol. Gen. Genet. 254, 495–504.
10.1007/s004380050444
CAS PubMed Web of Science® Google Scholar
[8] Bos, C.J., Debets, A.J.M., Swart, K., Huybers, A., Kobus, G., Slakhorst, S.M. (1988) Genetic analysis and construction of master strains for assignment of genes to six linkage groups in Aspergillus niger. Curr. Genet. 14, 437–443.
10.1007/BF00521266
CAS PubMed Web of Science® Google Scholar
[9] Bos, C.J., Slakhorst, S.M., Debets, A.J.M., Swart, K. (1993) Linkage group analysis in Aspergillus niger. Appl. Microbiol. Biotechnol. 38, 742–745.
10.1007/BF00167138
CAS Web of Science® Google Scholar
[10] van Hartingsveldt, W., Mattern, I.E., van Zeijl, C.M.J., Pouwels, P.H., van den Hondel, C.A.M.J.J. (1987) Development of a homologous transformation system for Aspergillus niger based on the pyrG gene. Mol. Gen. Genet. 206, 7–75.
10.1007/BF00326538
Google Scholar
[11] Pontecorvo, G., Roper, J.A., Hemmons, L.M., MacDonald, K.D., Bufton, A.W. (1953) The genetics of Aspergillus nidulans. Adv. Genet. 5, 141–238.
10.1111/j.1365-2958.2007.05940.x
CAS PubMed Web of Science® Google Scholar
[12] McCully, K.S., Forbes, E. (1965) The use of p-fluorophenylalanine with master strains of Aspergillus nidulans for assigning genes to linkage groups. Genet. Res. 6, 352–359.
10.1017/S0016672300004249
CAS PubMed Web of Science® Google Scholar
[13] Cove, D.J. (1966) The induction and repression of nitrate reductase in the fungus, Aspergillus nidulans. Biochim. Biophys. Acta 113, 51–56.
10.1016/S0926-6593(66)80120-0
CAS PubMed Web of Science® Google Scholar
[14] Tilburn, J., Scazzocchio, C., Taylor, G.G., Zabicky-Zissman, J.H., Lockington, R.A., Davies, R.W. (1983) Transformation by integration in Aspergillus nidulans. Gene 26, 205–221.
10.1016/0378-1119(83)90191-9
CAS PubMed Web of Science® Google Scholar
[15] Kelly, J.M., Hynes, M.J. (1977) Increased and decreased sensitivity to carbon catabolite repression of enzymes of acetate metabolism in mutants of Aspergillus nidulans. Mol. Gen. Genet. 156, 87–92.
10.1007/BF00272256
CAS PubMed Web of Science® Google Scholar
[16] Stemple, C.J., Davis, M.A., Hynes, M.J. (1998) The facC gene of Aspergillus nidulans encodes an acetyl inducible carnitine acetyltransferase. J. Bacteriol. 180, 6242–6251.
CAS PubMed Web of Science® Google Scholar

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Volume178, Issue1

September 1999

Pages 35-37

The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans

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The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans

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