Expression of urease does not affect the ability of Bordetella bronchiseptica to colonise and persist in the murine respiratory tract
David J. McMillan
Department of Biological Sciences, University of Wollongong, Wollongong, N.S.W. 2522, Australia
Search for more papers by this authorEva Medina
Department of Microbial Pathogenicity and Vaccine Research, Division of Microbiology, GBF-National Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany
Search for more papers by this authorCarlos A. Guzmán
Department of Microbial Pathogenicity and Vaccine Research, Division of Microbiology, GBF-National Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany
Search for more papers by this authorCorresponding Author
Mark J. Walker
Department of Biological Sciences, University of Wollongong, Wollongong, N.S.W. 2522, Australia
*Corresponding author. Tel.: +61 (42) 213439; Fax: +61 (42) 214135, E-mail address: [email protected]Search for more papers by this authorDavid J. McMillan
Department of Biological Sciences, University of Wollongong, Wollongong, N.S.W. 2522, Australia
Search for more papers by this authorEva Medina
Department of Microbial Pathogenicity and Vaccine Research, Division of Microbiology, GBF-National Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany
Search for more papers by this authorCarlos A. Guzmán
Department of Microbial Pathogenicity and Vaccine Research, Division of Microbiology, GBF-National Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany
Search for more papers by this authorCorresponding Author
Mark J. Walker
Department of Biological Sciences, University of Wollongong, Wollongong, N.S.W. 2522, Australia
*Corresponding author. Tel.: +61 (42) 213439; Fax: +61 (42) 214135, E-mail address: [email protected]Search for more papers by this authorAbstract
To investigate the role played by urease during the Bordetella bronchiseptica infection process, the ability to colonise and persist in the mouse respiratory tract of a urease-negative B. bronchiseptica BB7865 and a BB7865 derivative constitutively expressing urease was compared with that of the wild-type strain. The results obtained showed that neither constitutive expression nor abolishment of urease activity had any significant effect on the course of B. bronchiseptica infection. Therefore, under our experimental conditions, urease is not essential for B. bronchiseptica to colonise and persist within the murine host.
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