Volume 151, Issue 1 pp. 17-21

ATP production during dissimilatory nitrate reduction in Azorhizobium caulinodans IRBG 46

K Siva Raju

Corresponding Author

K Siva Raju

Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India

Corresponding author. Present address: Division of Crop Chemistry and Soil Science, CTRI, Rajahmundry 533 105, India.Search for more papers by this author
N.D Sharma

N.D Sharma

Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India

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M.L Lodha

M.L Lodha

Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India

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Abstract

In Azorhizobium caulinodans IRBG 46 grown microaerobically in culture solution with nitrate as sole source of nitrogen, almost all the activity (>95%) of nitrate reductase (NR) was localized in the particulate fraction (P 250), while total activity of nitrite reductase (NiR) was present in the soluble fraction. Membrane-bound NR was found to be dissimilatory type generating ATP, the ATP/2e ratios for electron transfer from NADH and succinate to NO3 being 1.83 and 1.46, respectively.

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