Improved methods for purification of an enterotoxin produced by Bacillus cereus

Corresponding Author

Kunihiro Shinagawa

Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

¹Correspondence to: K. Shinagawa, Iwate University, Faculty of Agriculture, Department of Veterinary Medicine, 3-18-8 Ueda, Morioka, Iwate 020, Japan.Search for more papers by this author

Junichi Sugiyama,

Junichi Sugiyama

Denka Seiken Co. Ltd., Gosen, Niigata, Japan

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Tomoji Terada,

Tomoji Terada

Denka Seiken Co. Ltd., Gosen, Niigata, Japan

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Naonori Matsusaka,

Naonori Matsusaka

Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

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Shunji Sugii,

Shunji Sugii

Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan

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Kunihiro Shinagawa,

Corresponding Author

Kunihiro Shinagawa

Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

¹Correspondence to: K. Shinagawa, Iwate University, Faculty of Agriculture, Department of Veterinary Medicine, 3-18-8 Ueda, Morioka, Iwate 020, Japan.Search for more papers by this author

Junichi Sugiyama,

Junichi Sugiyama

Denka Seiken Co. Ltd., Gosen, Niigata, Japan

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Tomoji Terada,

Tomoji Terada

Denka Seiken Co. Ltd., Gosen, Niigata, Japan

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Naonori Matsusaka,

Naonori Matsusaka

Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

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Shunji Sugii,

Shunji Sugii

Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan

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First published: May 1991

https://doi.org/10.1111/j.1574-6968.1991.tb04626.x

Citations: 7

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Summary

An enterotoxin (ET) produced by Bacillus cereus was purified by ammonium sulfate precipitation followed by chromatography on SP-Sephadex C-25, chromatofocusing, and gel filtration on Sephacryl S-300. Purified ET was electrophoretically homogeneous with a molecular mass of 45 000 and with an isoelectric point of 5.5 It showed vascular permeability activity in rabbits, was lethal to mice, and caused fluid accumulation in mouse ligated intestinal loops. It showed cytotoxicity to Vero cells, but did not show any hemolytic or phospholipase C activities. These biological activities were found to be easily inactivated by heating at 56°C for 5 min, by digestion with trypsin and pepsin, and by exposure to pH below 3.0 or higher than 11.

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Volume80, Issue1

May 1991

Pages 1-6

Improved methods for purification of an enterotoxin produced by Bacillus cereus

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Improved methods for purification of an enterotoxin produced by Bacillus cereus

Summary

REFERENCES

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