Microbial biofilms associated with biliary stent clogging
Emilio Guaglianone
Department of Technologies and Health, Istituto Superiore di Sanità
Microbial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Rome, Italy
Search for more papers by this authorRita Cardines
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorClaudia Vuotto
Department of Technologies and Health, Istituto Superiore di Sanità
Microbial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Rome, Italy
Search for more papers by this authorRoberta Di Rosa
Chair of Clinical Immunology, 2nd Faculty of Medicine, ‘Sapienza’ University of Rome, Rome, Italy
Search for more papers by this authorValentina Babini
Department SAIFET, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorPaola Mastrantonio
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorGianfranco Donelli
Department of Technologies and Health, Istituto Superiore di Sanità
Microbial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Rome, Italy
Search for more papers by this authorEmilio Guaglianone
Department of Technologies and Health, Istituto Superiore di Sanità
Microbial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Rome, Italy
Search for more papers by this authorRita Cardines
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorClaudia Vuotto
Department of Technologies and Health, Istituto Superiore di Sanità
Microbial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Rome, Italy
Search for more papers by this authorRoberta Di Rosa
Chair of Clinical Immunology, 2nd Faculty of Medicine, ‘Sapienza’ University of Rome, Rome, Italy
Search for more papers by this authorValentina Babini
Department SAIFET, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorPaola Mastrantonio
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Search for more papers by this authorGianfranco Donelli
Department of Technologies and Health, Istituto Superiore di Sanità
Microbial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Rome, Italy
Search for more papers by this authorEditor: John Costerton
Abstract
Endoscopic stenting is a palliative approach for the treatment of diseases involving biliary obstruction. Its major limitation is represented by stent occlusion, followed by life-threatening cholangitis, often requiring stent removal and replacement. Although it has been suggested that microbial colonization of biliary stents could play a role in the clogging process, the so far available data, particularly on the role of anaerobic bacteria, are not enough for a comprehensive description of this phenomenon. Our study was focused on the analysis of 28 explanted biliary stents by culturing, denaturing gradient gel electrophoresis and scanning electron microscopy to identify all the aerobic/anaerobic bacteria and fungi involved in the colonization of devices and to verify the ability of isolated anaerobic bacterial strains to form a biofilm in order to better understand the mechanisms of stent clogging.
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