Research Article
Yeast communities associated with the bulk-soil, rhizosphere and ectomycorrhizosphere of a Nothofagus pumilio forest in northwestern Patagonia, Argentina
M. Cecilia Mestre,
Carlos A. Rosa,
Silvana V.B. Safar,
Diego Libkind,
Sonia B. Fontenla,
Corresponding Author
M. Cecilia Mestre
Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), INIBIOMA (Universidad Nacional del Comahue-CONICET), Río Negro, Argentina
Correspondence: M. Cecilia Mestre, Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), UNCO- INIBIOMA (Universidad Nacional del Comahue-CONICET), Quintral 1250, Bariloche (8400), Río Negro, Argentina. Tel.:+54 2944 428505; fax: +54 2944 423111; e-mail: [email protected]Search for more papers by this authorCarlos A. Rosa
Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
Search for more papers by this authorSilvana V.B. Safar
Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
Search for more papers by this authorDiego Libkind
Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), INIBIOMA (Universidad Nacional del Comahue-CONICET), Río Negro, Argentina
Search for more papers by this authorSonia B. Fontenla
Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), INIBIOMA (Universidad Nacional del Comahue-CONICET), Río Negro, Argentina
Search for more papers by this authorM. Cecilia Mestre,
Carlos A. Rosa,
Silvana V.B. Safar,
Diego Libkind,
Sonia B. Fontenla,
Corresponding Author
M. Cecilia Mestre
Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), INIBIOMA (Universidad Nacional del Comahue-CONICET), Río Negro, Argentina
Correspondence: M. Cecilia Mestre, Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), UNCO- INIBIOMA (Universidad Nacional del Comahue-CONICET), Quintral 1250, Bariloche (8400), Río Negro, Argentina. Tel.:+54 2944 428505; fax: +54 2944 423111; e-mail: [email protected]Search for more papers by this authorCarlos A. Rosa
Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
Search for more papers by this authorSilvana V.B. Safar
Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
Search for more papers by this authorDiego Libkind
Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), INIBIOMA (Universidad Nacional del Comahue-CONICET), Río Negro, Argentina
Search for more papers by this authorSonia B. Fontenla
Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche (CRUB), INIBIOMA (Universidad Nacional del Comahue-CONICET), Río Negro, Argentina
Search for more papers by this authorAbstract
Soil microorganisms play an important role in soil quality and they interact closely with vegetation. Little is known about yeast diversity and function in forest soil ecosystems and their interactions with other biotic soil components, particularly in the mycorrhizosphere. We studied the diversity of yeasts inhabiting the bulk-soil, rhizosphere and ectomycorrhizosphere of a Nothofagus pumilio forest in Nahuel Huapi National Park (Bariloche, Argentina). Ectomycorrhizal infection was observed in all N. pumilio trees studied. A total of 126 yeast isolates were obtained, including 18 known and three possibly new species. Basidiomycetous yeasts were predominant in all soil fractions, and the most frequently isolated species was Cryptococcus podzolicus. Diversity indices and multivariate analyses were used to study and compare yeast communities in the bulk-soil, rhizosphere and ectomycorrhizosphere. Yeasts able to ferment glucose were found associated with the rhizosphere. Many of the recovered yeast species were associated with lignocelluloses compound degradation, which suggest that yeast plays an important role as a decomposer in these forest soils. Each soil fraction has a distinct yeast assemblage related to their physiologic capacities and soil nutrient availability.
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