Additional information and reprint requests:
Lori K. Hennessy, Ph.D.
Life Technologies Corporation
850 Lincoln Centre Drive
Mail Stop 404-1
Foster City, CA 94404
E-mail: [email protected]

^†

Presented in part at the 62nd Annual Scientific Meeting of the American Academy of Forensic Sciences, February 22-27, 2010, Seattle WA. Some of the data is also presented in the PrepFiler Express™ and PrepFiler Express BTA™ Forensic DNA Extraction Kit User Manual.

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Abstract

Abstract: The AutoMate Express™ Forensic DNA Extraction System was developed for automatic isolation of DNA from a variety of forensic biological samples. The performance of the system was investigated using a wide range of biological samples. Depending on the sample type, either PrepFiler™ lysis buffer or PrepFiler BTA™ lysis buffer was used to lyse the samples. After lysis and removal of the substrate using LySep™ column, the lysate in the sample tubes were loaded onto AutoMate Express™ instrument and DNA was extracted using one of the two instrument extraction protocols. Our study showed that DNA was recovered from as little as 0.025 μL of blood. DNA extracted from casework-type samples was free of detectable PCR inhibitors and the short tandem repeat profiles were complete, conclusive, and devoid of any PCR artifacts. The system also showed consistent performance from day-to-day operation.

References

1 Jeffreys AJ, Wilson V, Thein SL. Hypervariable ‘minisatellite’ regions in human DNA. Nature 1985; 314(6006): 67–73.
10.1038/314067a0
CAS PubMed Web of Science® Google Scholar
2 Jeffreys AJ, Wilson V, Thein SL. Individual-specific ‘fingerprints’ of human DNA. Nature 1985; 316(6023): 76–9.
10.1038/316076a0
CAS PubMed Web of Science® Google Scholar
3 Edwards A, Civitello A, Hammond HA, Caskey CT. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 1991; 49: 746–56.
CAS PubMed Web of Science® Google Scholar
4 Roewer L, Epplen JT. Rapid and sensitive typing of forensic stains by PCR amplification of polymorphic simple repeat sequences in case work. Forensic Sci Int 1992; 53: 163–71.
10.1016/0379-0738(92)90193-Z
CAS PubMed Web of Science® Google Scholar
5 Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Smerick JB, Budowle B. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. J Forensic Sci 2001; 46: 647–60.
10.1520/JFS15018J
CAS PubMed Web of Science® Google Scholar
6 Kochl S, Niederstatter H, Parson W. DNA extraction and quantitation of forensic samples using the phenol-chloroform method and real-time PCR. Methods Mol Biol 2005; 297: 13–30.
CAS PubMed Google Scholar
7 Walsh PS, Metzger DA, Higuchi R. Chelex^® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 1991; 10: 506–13.
CAS PubMed Web of Science® Google Scholar
8 Brevnov MG, Pawar HS, Mundt J, Calandro LM, Furtado MR, Shewale JG. Developmental validation of the PrepFiler Forensic DNA Extraction Kit for extraction of genomic DNA from biological samples. J Forensic Sci 2009; 54: 599–607.
10.1111/j.1556-4029.2009.01013.x
CAS PubMed Web of Science® Google Scholar
9 Brevnov MG, Mundt J, Benfield J, Treat-Clemons L, Kalusche G, Meredith J, et al. Automated extraction of DNA from forensic sample types using the PrepFiler Automated Forensic DNA Extraction Kit. JALA 2009; 14: 294–302.
CAS Web of Science® Google Scholar
10 Barbisin M, Fang R, O’Shea CE, Calandro LM, Furtado MR, Shewale JG. Developmental validation of the Quantifiler Duo DNA Quantification kit for simultaneous quantification of total human and human male DNA and detection of PCR inhibitors in biological samples. J Forensic Sci 2009; 54: 305–19.
10.1111/j.1556-4029.2008.00951.x
CAS PubMed Web of Science® Google Scholar
11 Applied Biosystems. AmpFℓSTR® Identifiler® PCR amplification kit user’s manual, part # 4323291 Rev. B. Foster City, CA: Applied Biosystems, 2001.
Google Scholar
12 Applied Biosystems. AmpFℓSTR® Identifiler® Plus PCR amplification kit user guide, part #: 4440211. Foster City, CA: Applied Biosystems, 2010.
Google Scholar
13 Applied Biosystems. AmpFℓSTR® MiniFiler™ PCR amplification kit user guide, part #: 4374618. Foster City, CA: Applied Biosystems, 2007.
Google Scholar
14 Gill P, Jeffreys AJ, Werrett DJ. Forensic application of DNA ‘fingerprints.’ Nature 1985; 318(6046): 577–9.
10.1038/318577a0
CAS PubMed Web of Science® Google Scholar
15 Qiagen. EZ1 DNA investigator handbook. Valencia, CA: Qiagen, 2009, http://www.qiagen.com/literature/default.aspx?Term=?Language=EN?LiteratureType=1?ProductCategory=232?r=1281 (accessed April 30, 2009).
Google Scholar
16 Promega Corporation. DNA IQ™ casework sample kit for Maxwell® 16. Madison, WI: Promega Corporation, 2008.
Google Scholar
17 Promega Corporation. Bone extraction protocol to be used with the DNA IQ™ system. Madison, WI: Promega Corporation, 2008.
Google Scholar
18 Fang R, Liu JY, Kijenski HL, Benfield J, Wong A, Lagacé R, et al. The HID EVOlution system for automation of DNA quantification and short tandem repeat analysis. JALA 2010; 15: 65–73.
CAS Web of Science® Google Scholar
19 Scientific Working Group on DNA Analysis Methods (SWGDAM). Revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Forensic Sci Commun 2004; 6(3): 1–6. http://www.fbi.gov/hq/lab/fsc/backissu/july2004/standards/2004_03_standards02.htm#perfcheck (accessed November 24, 2010).
Google Scholar

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Volume57, Issue4

July 2012

Pages 1022-1030

AutoMate Express™ Forensic DNA Extraction System for the Extraction of Genomic DNA from Biological Samples*

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AutoMate Express™ Forensic DNA Extraction System for the Extraction of Genomic DNA from Biological Samples*

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