Quantitative Analysis of Gamma-Hydroxybutyrate at Endogenous Concentrations in Hair using Liquid Chromatography Tandem Mass Spectrometry

Phillip A. Stout M.S.

Forensic Science Program, College of Criminal Justice, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341.

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Kelsie D. Simons M.S.,

Kelsie D. Simons M.S.

Forensic Science Program, College of Criminal Justice, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341.

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Sarah Kerrigan Ph.D.,

Sarah Kerrigan Ph.D.

Forensic Science Program, College of Criminal Justice, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341.

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Phillip A. Stout M.S.,

Phillip A. Stout M.S.

Forensic Science Program, College of Criminal Justice, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341.

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Kelsie D. Simons M.S.,

Kelsie D. Simons M.S.

Forensic Science Program, College of Criminal Justice, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341.

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Sarah Kerrigan Ph.D.,

Sarah Kerrigan Ph.D.

Forensic Science Program, College of Criminal Justice, Sam Houston State University, Box 2525, 1003 Bowers Blvd, Huntsville, TX 77341.

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First published: 01 March 2010

https://doi.org/10.1111/j.1556-4029.2009.01304.x

Citations: 29

Additional information and reprint requests:
Sarah Kerrigan, Ph.D.
Forensic Science Program Director
College of Criminal Justice
Sam Houston State University
Box 2525
1003 Bowers Blvd
Huntsville, TX 77341
Email: [email protected]

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Abstract

Abstract: A method capable of quantifying endogenous concentrations of gamma-hydroxybutyrate (GHB) in human head hair was developed and validated using liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS). Hair was digested under alkaline conditions, and GHB was isolated using liquid–liquid extraction. LC/MS/MS was performed using atmospheric pressure chemical ionization in the negative mode, multiple reaction monitoring, and deuterated internal standard (GHB-D₆). Linearity was observed between 0.1 and 100 ng/mg GHB (R² = 1.000). The limits of detection and quantitation in human hair were 0.2 and 0.4 ng/mg, respectively. Accuracy at 2 ng/mg and 10 ng/mg was determined to be 97% and 94%, and intra-assay CVs at these concentrations were 5.2% and 7.4% (n = 4). Beta-hydroxybutyrate (BHB), alpha-hydroxybutyrate, gamma-butyrolactone, and 1,4-butanediol did not produce an interference, and there was negligible ion suppression or enhancement from the matrix.

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Volume55, Issue2

March 2010

Pages 531-537

Quantitative Analysis of Gamma-Hydroxybutyrate at Endogenous Concentrations in Hair using Liquid Chromatography Tandem Mass Spectrometry

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Quantitative Analysis of Gamma-Hydroxybutyrate at Endogenous Concentrations in Hair using Liquid Chromatography Tandem Mass Spectrometry

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