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Ultrastructural Localization of Acid Phosphatase in Feeding Tokophrya infusionum*
MARIA A. RUDZINSKA,
MARIA A. RUDZINSKA
The Rockefeller University, New York
It is a great pleasure to acknowledge the excellent technical assistance of Mr. Erminio Gubert, Mrs. Karin Lifter, and Mrs. Sondra Lewengrub.
Search for more papers by this authorMARIA A. RUDZINSKA,
MARIA A. RUDZINSKA
The Rockefeller University, New York
It is a great pleasure to acknowledge the excellent technical assistance of Mr. Erminio Gubert, Mrs. Karin Lifter, and Mrs. Sondra Lewengrub.
Search for more papers by this author†
This investigation was supported by Research Grant AI08989-02, from NIAID, U. S. Public Health Service.
SYNOPSIS
A combined cytochemical and electronmicroscopic study of feeding Tokophrya revealed that it has 2 sources of acid phosphatase. One is from the prey, Tetrahymena, supplying newly formed food vacuoles with large amounts of enzyme. The other source is in Tokophrya itself, the enzyme being found in small vesicles, small dense elongate bodies surrounded by a membrane, or in residue vacuoles. It seems that the 2 former small structures contain insignificantly small amounts of phosphatase; however, large deposits of lead phosphate are present in residue vacuoles, former food vacuoles. Since Tokophrya has no cytopyge these vacuoles are not excreted. On the contrary, when feeding is resumed, they merge with food vacuoles, presumably supplying them with acid phosphatase. Whether this enzyme ultimately is derived from the prey Tetrahymena and persists undegraded in the residue vacuoles, or whether it is synthesized by Tokophrya cannot be determined from present work.
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