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Abnormal Infraciliatures of Balantidium coli and B. caviae (?) and Some Morphological Observations on These Species*
SERHIJ KRASCHENINNIKOW,
SERHIJ KRASCHENINNIKOW
Zoological Laboratory, University of Pennsylvania, Philadelphia, Pa.
Search for more papers by this authorSERHIJ KRASCHENINNIKOW,
SERHIJ KRASCHENINNIKOW
Zoological Laboratory, University of Pennsylvania, Philadelphia, Pa.
Search for more papers by this author†
Research supported by U. S. Public Health Grants, R. G. 4527 and E-999 (C).
Abstract
SYNOPSIS. Balantidium coli was cultured for 4 months in Dobell-Laidlaw's medium. About 10 specimens of monsters were found in the preparations (Chatton-Lwoff silver impregnation technique). One individual showed a tendency to polystomy. One doublet of Balantidium caviae (?) in preparations treated by the same technique was found in a population from a guinea pig.
The monsters of Balantidium coli are either single distomic individuals or doublets (homopolar or heteropolar).
The infraciliature of the monsters shows different degrees of deviation from the normal pattern. No reorganization field during the formation of additional cytostomes was observed, which agrees with the data on normal stomatogenesis. This process is very similar in both normal specimens and monsters and is characterized by the arrangement of radiating kineties surrounding the anlage of the cytostome and, evidently, by invagination of this area which later forms the peristome. The fate of single monsters and that of homopelar doublets is unknown. The heteropolar monsters are considered non-viable because the arrangement of the kineties in both presumptive daughters contradicts the rule of desmodexy (Chatton-Lwoff).
The occurrence of the monsters in cultures may possibly be explained by the action of acriflavine which is mutagenic for Escherichia coli, or by gentian-violet which inhibits fission of sea urchin eggs.
This paper apparently constitutes the first presentation of anomalies of the infraciliature in parasitic ciliates.
In addition the exact arrangement of the kineties and kinetosomes at the posterior pole of Balantidium coli is described.
REFERENCES
- 1 Balbiani, E. G. 1891 Sur la formation des monsters doubles chez les Infusoires ciliés. J. Anat. 27, 169–96.
- 2 Balbiani, E. G. 1893 Nouvelles recherches expérimentales sur la mérotomie des Infusoires ciliés. II. Ann. Micrograph. 5, 1–25; 49–84; 113–37.
- 3 Borowski, W. M. 1922 Zur Biologie der Infusorien. Arkhiv Russ. Protistol. 1, 136–40.
- 4 Calkins, G. N. 1913 E Biol. Bull. 21, 1911.
- 5 Calkins, G. N. 1925 Uroleptus mobilis V. The history of a double organism. J. Exptl. Zool. 41, 191–213.
- 6 Chatton, E. 1921 Reversion de la scission chez les ciliés. Réalisation d'individus distomes et polyenérgides des Glaucoma scintillans, se multipliant indéfinement par scissparité. Compt. rend. Acad. Sci. 173, 393–5.
- 7 Chatton, E. 1925 L'Action des facteurs externes sur les Infusoires. Le déterminisme de la formation des chaines chez les Colpidium. Compt. rend. Acad. Sci. 180, 1225–7.
- 8 Chatton, E. & Chatton, Mme. 1925 L'Action des facteurs externes sur la sexualité des Infusoires. Bactéries zygogènes et azygogènes. Compt. rend. soc. biol. 93, 675–8.
- 9 Chatton, E. & Chatton, Mme. 1925 L'Action des facteurs externes sur les Infusoires. Le déterminisme de la formation des chaines (dystomie) chez les Colpidium. Rev. Suisse Zool. 32, 99.
- 10 Chatton, E. & Lwoff, A. 1935a. Les Ciliés apostomes. Arch. zool. exptl. et gén. 77, 1–453.
- 11 Chatton, E. & Lwoff, A. 1935b. La constitution primitive de la strie ciliaire des infusoires. La desmodexie. Compt. rend. soc. biol. 118, 1068–72.
- 12 Collin, B. 1909 Sur les formes hypertrophiques et la croissance dégénerative chez quelques Acinétiens. Compt. rend. Acad. Sci. 742–5.
- 13 Collin, B. 19111912. Étude monographique sur les Acinétiens. I. Recherches expérimentales sur l'étendue des variations et les facteurs tératogènes. Arch. zool. exptl. et gén. 8, 421–97.
- 14 Corliss, J. O. 1953 Naturally-occurring” cases of homopolar doublets in Tetrahymena pyriformis. Proc. Soc. Protozool. 4, 4.
- 15 Corliss, J. O. 1953 Silver impregnation on ciliated protozoa by the Chatton-Lwoff technic. Stain Technol. 28, 2, 97–100.
- 16 Dawson, J. A. 1920 An experimental study of an amicronucleate Oxytricha. II. The formation of double animals or “twins”. J. Exptl. Zool. 30, 129–54.
- 17
De Garis, C. F.
1927
A genetic study of Paramecium caudatum in pure lines through an interval of experimentally induced monster formation.
J. Exptl. Zool.
49, 133–47.
10.1002/jez.1400490106 Google Scholar
- 18 Demerec, M., Bertani, G. & Flint, J. 1951 A survey of chemicals for mutagenic action on Escherichia coli. Am. Naturalist. 85, 119–36.
- 19 Dobell, C. & Laidlaw, P. 1926 On the cultivation of Entamoeba histolytica and some other entozoic amoebae. Parasitology 18, 293–318.
- 20 Dogiel, V. A. 1927 Monographie der Familie Ophryoscolecidae. Arch. Protistenk. 59, 1–288.
- 21 Druckrey, H., Schmahl, D. & Kaiser, K. 1957 Versuche mit zellteilungshemmenden Giften an unbefruchteten Seeigeleiern (Paracentrotus lividus und Spaerechinus granularis). Naturwissenschaften 44, 430–1.
- 22 Fauré-Fremiet, E. 1906 Sur un cas de monstruosité chez Stentor coeruleus. Arch. Anat. Microscop. 8, 660.
- 23 Fauré-Fremiet, E. 1945 Symétrie et polarité chez les Ciliés bi- ou multicomposites. Bull. Biol. 79, 106–50.
- 24 Fauré-Fremiet, E. 1948 Les mécanismes de la morphogénèse chez les Ciliés. Series B. Folia Biotheoretica, (De morphogenetica), 3, 25–58.
- 25 Fauré-Fremiet, E. 1948 Doublets homopolaires et régulation morphogénétique chez les Ciliés Leucophrys patula. Arch. Anat. Microscop. Morphol. Exptl. 37, 183–203.
- 26 Fauré-Fremiet, E. 1953 La bipartition énantiotrope chez les Ciliés Oligotriches. Arch. Anat. Microscop. Morphol. Exptl. 42, 209–225.
- 27 Herzfeld, E. 1925 Über das Vorkommen von Missbildungen und Monstrositäten bei Paramaecium spez. nebst einigen experimentellen Untersuchungen über deren Bedeutung. Jenaische Z. Naturwiss. 62, 81.
- 28 Hinrichs, M. A. 1928 Ultraviolet radiation and division in Paramecium caudatum. Physicl. Zool. 1, 394.
- 29 Jennings, H. S. 1908 Heredity, variation and evolution in Protozoa. I. The fate of new structural characters in Paramecium, with special reference to the question of the inheritance of acquired characters in Protozoa. J. Exptl. Zool. 5, 577–632.
- 30 Jennings, H. S. 1908 Heredity, variation and evolution in Protozoa. II. Heredity and variation in size and form in Paramecium, with studies of growth, environmental action and selection. Proc. Am. Phil. Soc. 47, 393–546.
- 31
Kimball, R. F.
1941
Double animals and amicronucleate animals in Euplotes patella, with particular reference to their conjugation.
J. Exptl. Zool.
86, 132.
10.1002/jez.1400860102 Google Scholar
- 32 Klein, M. 1938 Miss-bzw. Doppelbildungen am Silberliniensystem von Ciliaten. Arch. Protistenk 90, 292–8.
- 33 Klein, M. 1955 Potenzen erster nervlicher Differenzierungen. Acta neurovegetativa. 12, 1–23.
- 34 Klein, M. 1956 Form und Formänderung bei ciliaten Infusorien. Mikrokosmos 45, 203–8.
- 35 Krascheninnikow, S. 1955s J. Protozool. 3, (suppl.) 2.
- 36 Krascheninnikow, S. & Wenrich, D. H. 1957 F J. Protozool. 4, (suppl.) 11.
- 37
Krascheninnikow, S.
1958
An improved method for purifying Balantidia.
J. Parasitol.
44, 126–7.
10.2307/3274843 Google Scholar
- 38 Lom, J. 1954 Pathologische Veränderungen im Makronucleus des Infusorium Nyctotherus cordiformis Stein in der Kultur. Věstnik Českoslov. Společnosti. 19, 163–74.
- 39 Lom, J. 1957 A contribution to the morphology of the ciliates Balantidium elongatum and B. Duodeni. Českoslov. Parasitologie 4, 209–17.
- 40 MacDougall, S. 1929 Modifications in Chilodon uncinatus produced by ultraviolet radiation. J. Exptl. Zool. 54, 95–104.
- 41 MacDougall, S. 1931 Another mutation of Chilodon uncinatus produced by ultraviolet radiation with a description of its maturation process. J. Exptl. Zool. 58, 229–35.
- 42 McDonald, J. D. 1922 O Publ. Zoöl. 20, 243–300.
- 43 Pick-Levontin, E. M. & Cheissin, E. M. 1940 The variability of infusoria Balantidium coli from man and pig. Zool. Zhur. 19, 99–118.
- 44 Poljansky, G. & Strelkow, A. 1938 Étude expérimental sur variabilité de quelques Ophryoscolécidés. Arch. zool. exptl. et gén. 80, 1–123.
- 45 Popoff, M. 1909 Experimentalle Zellstudien, II. Arch. Zellforsch. 3, 125–80.
- 46
Pritze, F.
1928
Beiträge zur Kenntnis des Balantidium coli. Das Balantidium des Schweines in seiner Beziehung zum menschlichen Balantidium und sein Verhalten unter natürlichen und künstlichen Bedingungen.
Z. Parasitenk.
1, 345–415.
10.1007/BF02120381 Google Scholar
- 47 Puytorac, P. De 1952 Monstruosités naturelles chez Paramecium caudatum (Ehrb.) Holotriche Trichostome. Compt. rend. Anatomistes. 39 Réunion. Clermont-Ferrand, 7–9; 1–7.
- 48 Puytorac, P. De 1954 Contribution a l'étude cytologique et taxonomique des infusoires Astomes. Ann. Sci. nat. Zool., 11e serie, 85–270.
- 49 Sonneborn, T. M. 1932 E Biol. Bull. 53, 2, 63–187.
- 50 Weisz, B. 1954 Morphogenesis in Protozoa. Quart. Rev. Biol. 29, 207–29.
- 51 Witkin, E. M. 1947 Mutations in Escherichia coli induced by chemical agents. Cold Spring Harbor Symposia Quant. Biol. 12, 256–69.
- 52 Yagiu, R. 1956 Studies on the morphogenesis in Ciliata I. The regulation of the silver line system. J. Sci. Hiroshima Univ., Ser. B, Div. 1, 16, 53–9.
- 53 Yagiu, R. 1956 Studies on the morphogenesis in Ciliata II. The individuality of the macronucleus in maintenance of the peristome. J. Sci. Hiroshima Univ., Ser. B, Div. 1, 16, 61–72.
