Lipidomic analysis of platelet senescence
Katharina Ruebsaamen
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorGerhard Liebisch
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorAlfred Boettcher
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorGerd Schmitz
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorKatharina Ruebsaamen
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorGerhard Liebisch
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorAlfred Boettcher
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorGerd Schmitz
From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Search for more papers by this authorThis work was supported by Deutsche Forschungsgemeinschaft (SFB-TR 13/A3), related to “The European Lipidomics Initiative; shaping the life sciences,” a specific support action subsidized by the EC 2006-07 (Proposal 013032). This work was also supported by the seventh framework program of the EU-funded “LipidomicNet” (Lipid droplets as dynamic organelles of fat deposition and release: translational research towards human disease; Proposal 202272).
Abstract
BACKGROUND: A hallmark of platelet (PLT) storage lesion is the loss of PLT lipids. Due to technical limitations a detailed lipidomic analysis of plateletpheresis products during storage was so far not available.
STUDY DESIGN AND METHODS: Fifty plateletpheresis products were stored for 5 days at 22°C under agitation. Each day plasma and PLTs were isolated by gel filtration and lipid species analyzed by electrospray ionization tandem mass spectrometry.
RESULTS: During 5 days of storage the total lipid content decreased by 10% in PLTs and increased by 5% in plasma. We observed the following changes in lipid class fractions during storage relative to the day of preparation: increases of 69% ceramide (Cer), 32% lysophosphatidylcholine (LPC), and 49% cholesteryl esters (CE) and a decrease of 10% free cholesterol (FC) in PLTs and elevation of 43% LPC and 14% CE and a decline of 20% phosphatidylcholine (PC) and 24% FC in plasma. Significant lipid species shifts were observed for phosphatidylserine, Cer, and LPC. Correlation analysis of lipid changes in plasma indicated that lecithin-cholesterol-acyltransferase (LCAT) activity may be responsible for the shift in plasma lipid composition. These lipid changes correlated between plasma and PLTs for LPC, FC, and CE fractions.
CONCLUSIONS: This study presents for the first time detailed lipid species profiles of PLTs and plasma during storage of PLT concentrates. These data provide clear evidence for LCAT-mediated esterification of FC and LPC generation in the plasma of PLT concentrates. Moreover, we showed evidence that these changes also impact PLT lipid composition.
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