Volume 36, Issue 1 pp. E1-E20

The Kinetics of Cardiopulmonary Bypass: A Dual-Platform Proteomics Study of Plasma Biomarkers in Pediatric Patients Undergoing Cardiopulmonary Bypass

Todd M. Umstead

Todd M. Umstead

Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, and Pediatric Cardiovascular Research Center, the Department of Pediatrics

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Chia-jung K. Lu

Chia-jung K. Lu

Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, and Pediatric Cardiovascular Research Center, the Department of Pediatrics

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Willard M. Freeman

Willard M. Freeman

Departments of Pharmacology

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John L. Myers

John L. Myers

Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, and Pediatric Cardiovascular Research Center, the Department of Pediatrics

Surgery

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J. Brian Clark

J. Brian Clark

Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, and Pediatric Cardiovascular Research Center, the Department of Pediatrics

Surgery

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Neal J. Thomas

Neal J. Thomas

Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, and Pediatric Cardiovascular Research Center, the Department of Pediatrics

Public Health Sciences

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Nikolina Icitovic

Nikolina Icitovic

Public Health Sciences

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Vernon M. Chinchilli

Vernon M. Chinchilli

Public Health Sciences

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Akif Ündar

Corresponding Author

Akif Ündar

Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, and Pediatric Cardiovascular Research Center, the Department of Pediatrics

Surgery

Bioengineering, Pennsylvania State University College of Medicine, Hershey, PA, USA

Dr. Akif Ündar, Professor of Pediatrics, Surgery and Bioengineering, Penn State Hershey College of Medicine, 500 University Drive, Hershey, PA 17033, USA. E-mail: [email protected]Search for more papers by this author
David S. Phelps

David S. Phelps

Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, and Pediatric Cardiovascular Research Center, the Department of Pediatrics

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First published: 18 January 2012
Citations: 12

The senior authors, A.Ü. and D.S.P., contributed equally to this work.

Abstract

Abstract: This study was designed to investigate the expression kinetics and patterns of plasma biomarkers throughout the pediatric cardiopulmonary bypass (CPB) procedure to help predict those patients most at risk for complications. This study sampled plasma from pediatric CPB patients at five time points before, during, and after CPB. A dual-platform proteomics approach was then utilized which incorporated two-dimensional difference gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption ionization-time-of-flight/time-of-flight tandem mass spectrometry, and multi-analyte profile (MAP) assays to identify changes in expression of plasma protein biomarkers and characterize the patterns of these changes. A combined total of 134 proteins were identified with significant changes between the two platforms, with 53 coming from 2D-DIGE, 90 from MAP, and nine proteins that were identified using both methods. The proteins were then divided into 12 major groups based on the expression patterns, and two of the most clinically relevant proteins having the greatest changes in expression were selected from each group to use as “predictor biomarkers.” A potential model for prediction of patient outcome was then generated using these 24 proteins. The patterns of biomarker expression during pediatric CPB may provide insight into the prediction, prevention, or treatment of complications resulting from CPB, thereby helping to improve the outcomes of pediatric CPB patients and reduce the incidence of complications.

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