A Novel Strategy to Engineer Small-Diameter Vascular Grafts From Marrow-Derived Mesenchymal Stem Cells
Jie Zhao
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorLiangqi Liu
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorJing Wei
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorDongyang Ma
Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou, China
Search for more papers by this authorWenxin Geng
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorXingrong Yan
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorJun Zhu
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorHuicong Du
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorYing Liu
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorLiwen Li
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorCorresponding Author
Fulin Chen
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Prof. Fulin Chen, Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an 710069, P.R. China. E-mail: [email protected]Search for more papers by this authorJie Zhao
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorLiangqi Liu
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorJing Wei
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorDongyang Ma
Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou, China
Search for more papers by this authorWenxin Geng
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorXingrong Yan
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorJun Zhu
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorHuicong Du
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorYing Liu
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorLiwen Li
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Search for more papers by this authorCorresponding Author
Fulin Chen
Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an
Prof. Fulin Chen, Key Lab of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi' an 710069, P.R. China. E-mail: [email protected]Search for more papers by this authorAbstract
Tissue-engineered blood vessels have mainly relied on endothelial cells (ECs), smooth muscle cells (SMCs), and biocompatible materials. However, long-term results have revealed several material-related failures, such as stenosis, thromboembolization, and the risk of infection. Furthermore, SMCs from elderly persons have reduced capacity in proliferation and collagen production. Mesenchymal stem cells (MSCs) have the ability to differentiate into multiple cell lineages, including osteoblasts, chondrocytes, ECs, and SMCs. In the current experiment, rabbit MSCs were cultured to form a cell sheet. A tissue-engineered vascular graft (TEVG) was fabricated by rolling the MSC sheet around a mandrel. The TEVG was implanted into a defect of the common carotid artery after it was examined macroscopically and microscopically. Hematoxylin and eosin staining showed that cell sheet was composed of five to seven layers of cells with the thickness of 40–50 µm. Results from the adhesion assay revealed that MSCs had similar antiplatelet adhesion property to ECs. Histological analysis of TEVGs showed that the layers of the cell sheet had fully fused in vitro. After implantation, TEVGs had excellent patency and integrated well with the native vessel. The structure of the TEVGs was similar to that of the native artery 4 weeks after implantation. Electron microscopy showed that the implanted TEVGs endothelialized. These results indicated that a completely biological TEVG could be assembled with autologous MSCs. These TEVGs are useful for revascularization in humans, which would reduce the occurrence of complications caused by foreign materials.
REFERENCES
- 1 Roh JL, Kim MR, Choi SH, et al. Can patients with head and neck cancers invading carotid artery gain survival benefit from surgery? Acta Otolaryngol 2008; 128: 1370–4.
- 2 Hurtado-Lopez LM, Fink-Josephi G, Ramos-Mendez L, Dena-Espinoza E. Nonresectable carotid body tumor: hybrid surgical procedure to achieve complete and safe resection. Head Neck 2008; 30: 1646–9.
- 3 Pons Y, Ukkola-Pons E, Clement P, Baranger B, Conessa C. Carotid artery resection and reconstruction with superficial femoral artery transplantation: a case report. Head Neck Oncol 2009; 1: 19.
- 4 Feiz-Erfan I, Han PP, Spetzler RF, et al. Salvage of advanced squamous cell carcinomas of the head and neck: internal carotid artery sacrifice and extracranial-intracranial revascularization. Neurosurg Focus 2003; 14: e6.
- 5 Chazono H, Okamoto Y, Matsuzaki Z, et al. Extracranial-intracranial bypass for reconstruction of internal carotid artery in the management of head and neck cancer. Ann Vasc Surg 2003; 17: 260–5.
- 6 Muhm M, Grasl M, Burian M, Exadaktylos A, Staudacher M, Polterauer P. Carotid resection and reconstruction for locally advanced head and neck tumors. Acta Otolaryngol 2002; 122: 561–4.
- 7 Krumholz HM. Cardiac procedures, outcomes, and accountability. N Engl J Med 1997; 336: 1522–3.
- 8 Kannan RY, Salacinski HJ, Butler PE, Hamilton G, Seifalian AM. Current status of prosthetic bypass grafts: a review. J Biomed Mater Res B Appl Biomater 2005; 74: 570–81.
- 9 Tiwari A, Salacinski H, Seifalian AM, Hamilton G. New prostheses for use in bypass grafts with special emphasis on polyurethanes. Cardiovasc Surg 2002; 10: 191–7.
- 10 Van Damme H, Deprez M, Creemers E, Limet R. Intrinsic structural failure of polyester (Dacron) vascular grafts. A general review. Acta Chir Belg 2005; 105: 249–55.
- 11 Dearani JA, Danielson GK, Puga FJ, et al. Late follow-up of 1095 patients undergoing operation for complex congenital heart disease utilizing pulmonary ventricle to pulmonary artery conduits. Ann Thorac Surg 2003; 75: 399–410; discussion -1.
- 12 Weinberg CB, Bell E. A blood vessel model constructed from collagen and cultured vascular cells. Science 1986; 231: 397–400.
- 13 Allwood MC, Martin H. Factors influencing the stability of ranitidine in TPN mixtures. Clin Nutr 1995; 14: 171–6.
- 14 L'Heureux N, Paquet S, Labbe R, Germain L, Auger FA. A completely biological tissue-engineered human blood vessel. FASEB J 1998; 12: 47–56.
- 15 Seliktar D, Black RA, Vito RP, Nerem RM. Dynamic mechanical conditioning of collagen-gel blood vessel constructs induces remodeling in vitro. Ann Biomed Eng 2000; 28: 351–62.
- 16 Tschoeke B, Flanagan TC, Koch S, et al. Tissue-engineered small-caliber vascular graft based on a novel biodegradable composite fibrin-polylactide scaffold. Tissue Eng Part A 2009; 15: 1909–18.
- 17 Gauvin R, Ahsan T, Larouche D, et al. A novel single-step self-assembly approach for the fabrication of tissue-engineered vascular constructs. Tissue Eng Part A 2009; 16: 1737–47.
- 18 Kassem M, Burns J, Abd-Allah B. Blood vessels engineered from human cells. Lancet 2005; 366: 891–2; author reply 2-3.
- 19 Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284: 143–7.
- 20 Zhao LR, Duan WM, Reyes M, Keene CD, Verfaillie CM, Low WC. Human bone marrow stem cells exhibit neural phenotypes and ameliorate neurological deficits after grafting into the ischemic brain of rats. Exp Neurol 2002; 174: 11–20.
- 21 Hashi CK, Zhu Y, Yang GY, et al. Antithrombogenic property of bone marrow mesenchymal stem cells in nanofibrous vascular grafts. Proc Natl Acad Sci USA 2007; 104: 11915–20.
- 22 Matsumura G, Miyagawa-Tomita S, Shin'oka T, Ikada Y, Kurosawa H. First evidence that bone marrow cells contribute to the construction of tissue-engineered vascular autografts in vivo. Circulation 2003; 108: 1729–34.
- 23 Sittinger M, Hutmacher DW, Risbud MV. Current strategies for cell delivery in cartilage and bone regeneration. Curr Opin Biotechnol 2004; 15: 411–8.
- 24 Raghunath J, Rollo J, Sales KM, Butler PE, Seifalian AM. Biomaterials and scaffold design: key to tissue-engineering cartilage. Biotechnol Appl Biochem 2007; 46: 73–84.
- 25 Asplund B, Sperens J, Mathisen T, Hilborn J. Effects of hydrolysis on a new biodegradable co-polymer. J Biomater Sci Polym Ed 2006; 17: 615–30.
- 26 Kikuchi A, Okano T. Nanostructured designs of biomedical materials: applications of cell sheet engineering to functional regenerative tissues and organs. J Control Release 2005; 101: 69–84.
- 27 Mimura T, Amano S, Yokoo S, et al. Tissue engineering of corneal stroma with rabbit fibroblast precursors and gelatin hydrogels. Mol Vis 2008; 14: 1819–28.
- 28 Yang J, Yamato M, Kohno C, et al. Cell sheet engineering: recreating tissues without biodegradable scaffolds. Biomaterials 2005; 26: 6415–22.
- 29 Zhou Y, Chen F, Ho ST, Woodruff MA, Lim TM, Hutmacher DW. Combined marrow stromal cell-sheet techniques and high-strength biodegradable composite scaffolds for engineered functional bone grafts. Biomaterials 2007; 28: 814–24.
- 30 Chen F, Zhou Y, Barnabas ST, Woodruff MA, Hutmacher DW. Engineering tubular bone constructs. J Biomech 2007; 40(Suppl 1): S73–9.
- 31 Ng KW, Hutmacher DW. Reduced contraction of skin equivalent engineered using cell sheets cultured in 3D matrices. Biomaterials 2006; 27: 4591–8.
- 32 L'Heureux N, McAllister TN, de la Fuente LM. Tissue-engineered blood vessel for adult arterial revascularization. N Engl J Med 2007; 357: 1451–3.
- 33 L'Heureux N, Dusserre N, Konig G, et al. Human tissue-engineered blood vessels for adult arterial revascularization. Nat Med 2006; 12: 361–5.
- 34 L'Heureux N, Stoclet JC, Auger FA, Lagaud GJ, Germain L, Andriantsitohaina R. A human tissue-engineered vascular media: a new model for pharmacological studies of contractile responses. FASEB J 2001; 15: 515–24.
- 35 Chen F, Mao T, Tao K, Chen S, Ding G, Gu X. Bone graft in the shape of human mandibular condyle reconstruction via seeding marrow-derived osteoblasts into porous coral in a nude mice model. J Oral Maxillofac Surg 2002; 60: 1155–9.
- 36 von Smitten K. Breaking strength and suture holding capacity of syngeneic aortic vein grafts in the rat. Acta Chir Scand 1981; 147: 545–9.
Citing Literature
