Volume 16, Issue 2 pp. 119-123

Levofloxacin Susceptibility Testing for Helicobacter pylori in China: Comparison of E-Test and Disk Diffusion Method

Chaohui Yu,

Chaohui Yu

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Lan Li,

Lan Li

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Wenguo Chen,

Wenguo Chen

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Yangwen Jiao,

Yangwen Jiao

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Ningmin Yang,

Ningmin Yang

Hangzhou Zhiyuan Medical Inspection Institute, Hangzhou

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En Yang,

En Yang

Hangzhou Zhiyuan Medical Inspection Institute, Hangzhou

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Jianzhong Zhang,

Jianzhong Zhang

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

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Lihua Chen,

Lihua Chen

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Youming Li,

Youming Li

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Chaohui Yu,

Chaohui Yu

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Lan Li,

Lan Li

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Wenguo Chen,

Wenguo Chen

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Yangwen Jiao,

Yangwen Jiao

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Ningmin Yang,

Ningmin Yang

Hangzhou Zhiyuan Medical Inspection Institute, Hangzhou

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En Yang,

En Yang

Hangzhou Zhiyuan Medical Inspection Institute, Hangzhou

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Jianzhong Zhang,

Jianzhong Zhang

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

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Lihua Chen,

Lihua Chen

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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Youming Li,

Youming Li

Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou

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First published: 16 March 2011

https://doi.org/10.1111/j.1523-5378.2011.00820.x

Citations: 18

Reprints request to: Prof. Youming Li, The First Affiliated Hospital, College of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou 310003, China. E-mail: [email protected]

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Abstract

Background: The aims of this study were to compare disk diffusion with E-test method for levofloxacin susceptibility testing of Helicobacter pylori and standardized breakpoints for disk diffusion as a stable and reliable method for determining qualitative levofloxacin susceptibility.

Materials and Methods: We determined the levofloxacin susceptibility of 45 H. pylori strains isolated from Chinese patients by the E-test method. Disk diffusion was evaluated as an alternative method to determine susceptibility and compared with the E-test results by linear regression analysis.

Results: The minimum inhibitory concentration (MIC) values tested by E-test method ranged from 0.047 to 32 μg/mL. Resistance to levofloxacin was detected in 16 (35.6%) isolates. The levofloxacin disk zone sizes obtained by disk diffusion method correlated well (r² = .877) with the MICs obtained by E-test method. As a consequence of regression analysis, isolates with inhibition diameters <12 mm were considered resistant to levofloxacin. There was 100% agreement between the two methods for levofloxacin, applying the regression-based breakpoints.

Conclusions: The disk diffusion method is equivalent to the E-test method for testing levofloxacin susceptibility of H. pylori strains; it is more practical and inexpensive, and it is suitable for the analysis of a small number of isolates compared with the E-test method.

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Volume16, Issue2

April 2011

Pages 119-123

Levofloxacin Susceptibility Testing for Helicobacter pylori in China: Comparison of E-Test and Disk Diffusion Method

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Levofloxacin Susceptibility Testing for Helicobacter pylori in China: Comparison of E-Test and Disk Diffusion Method

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