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Accumulation of Arachidonic Acid Cyclo- and Lipoxygenase Products in Rat Brain During Ischemia and Reperfusion: Effects of Treatment with GM1-Lactone

Corresponding Author

A. Petroni

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

Address correspondence and reprint requests to Dr. A. Petroni at Institute of Pharmacological Sciences, University of Milan, Vila Bal-zaretti 9, 20133 Milan, Italy.Search for more papers by this author

A. Bertazzo,

A. Bertazzo

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

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S. Sarti,

S. Sarti

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

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C. Galli,

C. Galli

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

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A. Petroni,

Corresponding Author

A. Petroni

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

A. Bertazzo,

A. Bertazzo

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

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S. Sarti,

S. Sarti

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

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C. Galli,

C. Galli

Institute of Pharmacological Sciences, University of Milan, Milan. Italy

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First published: September 1989

https://doi.org/10.1111/j.1471-4159.1989.tb11768.x

Citations: 35

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Abstract

The aim of our study was to investigate the changes of various biochemical parameters (concentrations of lactate, free arachidonate, cyclo- and lipoxygenase products) in rat brain after ischemia and reperfusion and the effects of pre-treatment with the ganglioside derivative GM1-lactone on the same parameters. Ischemia was induced by reversible occlusion of common carotid arteries for 20 min, which in-cluded a final 5 min of respiration of 5% oxygen in nitrogen. Reperfusion was obtained by removing the occlusion. Pre-ischemic conditions were obtained on sham-operated animals. Animals were killed by microwave irradiation of their heads. Brain levels of lactate and of free arachidonate were markedly increased after ischemia and returned to normal values at 5 min of reperfusion. Levels of the cyclooxygenase metabolites prostaglandin F_2α, 6-keto-prostaglandin F_1α, and thromboxane B₂ were increased after ischemia, whereas levels of the lipoxygenase metabolite leukotriene C, (LTC₄) did not. change. After reperfusion, a very' marked increase of the cyclooxygenase products occurred but not of LTC₄. Treatment with GM1-lactone prevented the elevation of cyclo- and lipoxygenase metabolites especially during reperfusion, with limited effects on lactate and free arachidonate levels.

Abbreviations used:

AA: arachidonic acid
CO: cyclooxygenase
DAG: diacylglycerol
EIA: enzymatic immunoassay
FFA: free fatty acids
HETE: hydroxyeicosatetraenoic acid
LTC₄: leukotriene C₄
LO: lipoxygenase
PGF_2α: prostaglandin F_2α; 6-keto-PGF_1α, 6-keto-prostaglandin F_1α
RIA: radioimmunoassay
TXB₂: thromboxane B₂. Ganglioside nomenclature is according to Svennerholm (1970)

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Volume53, Issue3

September 1989

Pages 747-752

Accumulation of Arachidonic Acid Cyclo- and Lipoxygenase Products in Rat Brain During Ischemia and Reperfusion: Effects of Treatment with GM1-Lactone

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Accumulation of Arachidonic Acid Cyclo- and Lipoxygenase Products in Rat Brain During Ischemia and Reperfusion: Effects of Treatment with GM1-Lactone

Abstract

Abbreviations used:

REFERENCES

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