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Characteristics of Sorbitol Uptake in Rat Glial Primary Cultures
Bernd Stahl,
Heinrich Wiesinger,
Bernd Hamprecht,
Bernd Stahl
Physiologisch-chernisches Institut der Universität, Tübingen, F.R.G.
Search for more papers by this authorCorresponding Author
Heinrich Wiesinger
Physiologisch-chernisches Institut der Universität, Tübingen, F.R.G.
Address correspondence and reprint requests to Dr. H. Wiesinger at Physiologisch-chernisches Institut der Universität, Hoppe-Seyler-Str. 4, D-7400 Tübingen, F.R.G.Search for more papers by this authorBernd Hamprecht
Physiologisch-chernisches Institut der Universität, Tübingen, F.R.G.
Search for more papers by this authorBernd Stahl,
Heinrich Wiesinger,
Bernd Hamprecht,
Bernd Stahl
Physiologisch-chernisches Institut der Universität, Tübingen, F.R.G.
Search for more papers by this authorCorresponding Author
Heinrich Wiesinger
Physiologisch-chernisches Institut der Universität, Tübingen, F.R.G.
Address correspondence and reprint requests to Dr. H. Wiesinger at Physiologisch-chernisches Institut der Universität, Hoppe-Seyler-Str. 4, D-7400 Tübingen, F.R.G.Search for more papers by this authorBernd Hamprecht
Physiologisch-chernisches Institut der Universität, Tübingen, F.R.G.
Search for more papers by this authorAbstract
Uptake of [U-14C]sorbitol was studied in astroglia-rich rat primary cultures. Initial rate of sorbitol uptake is proportional to sorbitol concentration between 20 μM and 400 mM. Sorbitol transport is not inhibited by glucose, fructose, and a variety of structurally related polyols, or by cy-tochalasin B, an inhibitor of glucose transport. Phloretin, phlorizin, filipin, and n-hexanol, all compound that alter the properties of biological membranes, and the sulfhydryl reagent p-chloromercuribenzoate inhibit sorbitol uptake to various degrees. Variation in the concentrations of extracellular Na+ and K+ does not affect transfer of sorbitol across the cell membrane. It is concluded that sorbitol is taken up into glial cells by a diffusion process, not involving a carrier and probably not through the lipid bilayer, but through a proteinaceous channel-like structure.
Abbreviations used:
-
- DMEM
-
- Dulbecco's modified Eagle's medium
-
- DTT
-
- dithiothreitol
-
- PBS
-
- phosphate-buffered saline
-
- PCMB
-
- p-chlo-romercuribenzoate
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