Volume 49, Issue 3 pp. 821-827

Full Access

Lack of Selectivity Between the Uptake of [³H] Adrenaline and [³H]Noradrenaline into Rat Hypothalamic Slices

C. Routledge,

C. Routledge

Department of Physiology and Pharmacology, Nottingham University Medical School, Nottingham, England

Search for more papers by this author

C. A. Marsden,

Corresponding Author

C. A. Marsden

Department of Physiology and Pharmacology, Nottingham University Medical School, Nottingham, England

Address correspondence and reprint requests to Prof. C. A. Marsden at Department of Physiology and Pharmacology, Medical School, Queen's Medical Centre, Clifton Boulevard, Nottingham NG7 2UH, U.K.Search for more papers by this author

C. Routledge,

C. Routledge

Department of Physiology and Pharmacology, Nottingham University Medical School, Nottingham, England

Search for more papers by this author

C. A. Marsden,

Corresponding Author

C. A. Marsden

Department of Physiology and Pharmacology, Nottingham University Medical School, Nottingham, England

First published: September 1987

https://doi.org/10.1111/j.1471-4159.1987.tb00967.x

Citations: 4

The present address of Dr. C. Routledge is Syntex Research, 3401 Hillview Avenue, P.O. Box 10850, Palo Alto, CA 94303.

Share a link

Email
Wechat
Bluesky

Abstract

Abstract: The uptake of [³H]adrenaline and [³H]- noradrenaline into rat hypothalamic slices was compared for determination of whether adrenaline uptake was independent of uptake into noradrenergic neurones. Kinetic analysis revealed a similar high-affinity uptake process for both adrenaline and noradrenaline, with K_m and V_max values within similar ranges. These uptakes were inhibited by desipramine and maprotiline in a dose-dependent manner, but the selective dopamine and 5-hydroxytryptamine up- take inhibitors benztropine and fluoxetine, respectively, were without effect. Competition for uptake sites by unlabelled adrenaline with [³H]adrenaline and [³H]- noradrenaline and by unlabelled noradrenaline with [³H] adrenaline and [³H]noradrenaline was very similar. Lesioning of the major adrenaline-containing cell group (C₁ cell group) decreased the hypothalamic adrenaline concentration but had no effect on hypothalamic [³H]adrenaline or [³H]noradrenaline uptake. The results suggest that exogenous adrenaline is largely taken up by high-affinity sites on noradrenergic nerve terminals.

Abbreviations used:

5-HT: 5-hydroxytryptamine
RVL: rostral ventral medulla

References

Armstrong D. M., Ross C. A., Pickel V. M., Joh T. H., and Reis P. J. (1982) Distribution of dopamine, noradrenaline and adrenaline containing cell bodies in rat medulla oblongata; demonstration by immunocytochemical localization of catecholamine biosynthetic enzymes. J. Comp. Neural. 212, 173–187.
10.1002/cne.902120207
CAS PubMed Web of Science® Google Scholar
Berger B. and Glowinski J. (1978) Dopamine uptake in serotoninergic terminals in vitro: a valuable tool for the histochemical differentiation of catecholaminergic and serotoninergic terminals in rat cerebral structures. Brain Res. 147, 29–45.
10.1016/0006-8993(78)90770-9
CAS PubMed Web of Science® Google Scholar
Bjorklund A. and Lindvall O. (1975) Dopamine in dendrites of substantia nigra neurones: suggestions for a role in dendritic terminals. Brain Res. 83, 531–537.
10.1016/0006-8993(75)90849-5
CAS PubMed Web of Science® Google Scholar
Brenneman D. E. and Rutledge C. O. (1979) Alteration of catecholamine uptake in cerebral cortex from rats fed a saturated fat diet. Brain Res. 179, 295–304.
10.1016/0006-8993(79)90445-1
CAS PubMed Web of Science® Google Scholar
Farnebo L. O., Fuxe K., Hamberger B., and Ljungdahl H. (1970) Effect of some antiparkinsonian drugs in catecholamine neurons. J. Pharm. Pharmacol. 22, 733–737.
10.1111/j.2042-7158.1970.tb08420.x
CAS PubMed Google Scholar
Feuerstein T. J. and Meriting G. (1985) False labelling of dopaminergic nerve endings by 0.1 μmol/l (³H) serotonin in the rabbit caudate nucleus. Naunyn Schmiedebergs Arch. Pharmacol 330 (Suppl 1), R66.
Google Scholar
Fujiwara H., Fujii Y., and Tanaka C. (1983) Labeled norepinephrine uptake and release in rat globus pallidus. Brain Res 279 127–132.
10.1016/0006-8993(83)90170-1
CAS PubMed Web of Science® Google Scholar
Fujiwara H., Fujii Y., Saijoh K., and Tanaka C. (1984) Evidence for the neurotransmitter role of norepinephrine in the ventral thalamic nucleus of the guinea pig: localization, uptake and release. Brain Res. 301, 131–137.
10.1016/0006-8993(84)90409-8
PubMed Web of Science® Google Scholar
Fuller R. W. (1982) Pharmacology of brain epinephrine neurons. Annu. Rev. Pharmacol. Toxicol. 22, 31–55.
10.1146/annurev.pa.22.040182.000335
CAS PubMed Web of Science® Google Scholar
Fuller R. W., Perry K. W., and Hemrick S. K. (1980) Pharmacological modification of adrenaline neurons in rat brain, in Central Adrenaline Neurons. Basic Aspects and Their Role in Cardiovascular Functions ( K. Fuxe, M. Goldstein, B. Hökfelt, and T. Hökfelt, eds), pp. 87–95. Pergamon Press, Oxford .
10.1016/B978-0-08-025927-7.50012-0
Google Scholar
Goshima Y., Kubo T., and Misu Y. (1985) Autoregulation of endogenous epinephrine release via presynaptic adrenoceptors in the rat hypothalamic slice. J. Pharmacol. Exp. Ther. 235, 248–253.
CAS PubMed Web of Science® Google Scholar
Hökfelt T., Fuxe K., Goldstein M., and Johansson O. (1974) Immunohistochemical evidence for the existence of adrenaline neurons in the rat brain. Brain Res. 66, 235–252.
10.1016/0006-8993(74)90143-7
CAS Web of Science® Google Scholar
Iversen L. L. (1967) The Uptake and Storage of Noradrenaline in Sympathetic Nerves. Cambridge University Press, London .
Google Scholar
Iversen L. L. (1975) Uptake processes for biogenic amines, in Handbook of Psychopharmacology ( L. L. Iversen, S. D. Iversen, and S. H. Snyder, eds), pp. 381–442. Plenum Press, New York .
Google Scholar
Kelly E., Jenner P., and Marsden C. D. (1985) Evidence that [³H]- dopamine uptake is taken up and released from non-dopaminergic nerve terminals in the rat substantia nigra in vitro. J. Neurochem. 45, 137–144.
10.1111/j.1471-4159.1985.tb05485.x
CAS PubMed Web of Science® Google Scholar
Kuhar M. J. (1973) Neurotransmitter uptake: a tool in identifying neurotransmitter-specific pathways. Life Sci. 13, 1623–1624.
10.1016/0024-3205(73)90110-0
CAS PubMed Web of Science® Google Scholar
Langer S. Z., Briley M., and Dubcovich M. L. (1980) Adrenergic receptor mechanisms in the central nervous system in relation to catecholamine neurons, in Central Adrenaline Neurons. Basic Aspects and Their Role in Cardiovascular Functions ( K. Fuxe, M. Goldstein, B. Hökfelt, and T. Hökfdt, eds), pp. 199–211.
Google Scholar
Maitre L., Staehlin M., and Bein H. J. (1971) Blockade of noradrenaline uptake by BA-34276—a new antidepressant drug. Biochem. Pharmacol. 20, 2169–2186.
10.1016/0006-2952(71)90216-4
CAS PubMed Web of Science® Google Scholar
Maitre L., Waldmeier P. C., Baumann P. A., and Staehlin M. (1974) Effects of maprotiline, a new anti-depressant drug, on serotonin uptake, in Advances in Biochemical Psychopharmacology, Vol. 10: Serotonin—New Vistas: Histochemistry and Pharmacology ( E. Costa, G. L. Gessa, and M. Sandier, eds), pp. 297–304. Raven Press, New York .
Google Scholar
Mefford I. N. (1981) Application of high performance liquid chromatography with electrochemical detection for neurochemical analysis measurement of catecholamines, serotonin and metabolites in rat brain. J. Neurosci. Methods 3, 207–224.
10.1016/0165-0270(81)90056-X
CAS PubMed Web of Science® Google Scholar
Mefford I. N., Roth K. A., Jurik S. M., Collman V., Mclntire S., Tolbert L., and Barchas J. D. (1985) Epinephrine accumulation in rat brain after chronic administration of pargyline and LY51641—comparison with other brain amines. Brain Res. 339, 342–345.
10.1016/0006-8993(85)90101-5
PubMed Web of Science® Google Scholar
Pellegrino L. J., Pellegrino A. S., and Cushman R. J. (1979), A Stereotaxis Atlas of the Rat Brain. Plenum Press, New York .
Google Scholar
Routledge C. and Marsden C. A. (1987) Electrical stimulation of the rostral ventrolateral medulla of the rat increases mean arterial pressure and adrenaline release in the posterior hypothalamus. Neuroscience 20, 457–466.
10.1016/0306-4522(87)90104-7
CAS PubMed Web of Science® Google Scholar
Scatton B. and Bartholini G. (1980) Effects of anti-hypertensive and other drugs on central adrenaline utilization, in Central Adrenaline Neurons. Basic Aspects and Their Role in Cardiovascular Function ( K. Fuxe, M. Goldstein, B. Hökfelt, and T. Hökfelt, eds), pp. 183–197. Pergamon Press, Oxford .
10.1016/B978-0-08-025927-7.50020-X
Google Scholar
Scatton B., Pelayo F., Dubocovich M. L., Langer S. Z., and Bartholini G. (1978) Effects of clondine on the cerebral adrenaline turnover and the adrenaline release in nucleus tractus solitarii of the rat, in Presynaptic Receptors ( S. Langer, K. Starke, and M. Dubocovich, eds), pp. 231–236. Pergamon Press, Oxford .
Google Scholar
Scatton B., Pelayo F., Dubocovich M. L., Langer S. Z., and Bartholini G. (1979) Effect of clonidine on utilization and potassium-evoked release of adrenaline in rat brain areas. Brain Res. 176, 197–201.
10.1016/0006-8993(79)90887-4
CAS PubMed Web of Science® Google Scholar
Shaskan E. G. and Snyder S. H. (1970) Kinetics of serotonin accumulation into slices from rat brain: relationship to catecholamine uptake. J. Pharmacol. Exp. Ther. 175, 404–418.
CAS PubMed Web of Science® Google Scholar
Tassin J. P., Thierry A. M., Blanc G., and Glowinski J. (1974) Evidence for a specific uptake of dopamine by dopaminergic terminals in the rat cerebral cortex. Naunyn Schmiedebergs Arch. Pharmacol. 282, 239–244.
10.1007/BF00501232
CAS PubMed Web of Science® Google Scholar
Tessel R. E., Kennedy L. E., Burgess S. K., and Borchardt R. T. (1978) Epinephrine in rat hypothalamus: antagonism by desipramine of 6-hydroxydopamine-induced depletion. Brain Res. 153, 615–617.
10.1016/0006-8993(78)90347-5
CAS PubMed Web of Science® Google Scholar
Wong D. T., Bymaster F. F., Horng, J. S., and Molloy B. B. (1975) A new selective inhibitor for uptake of serotonin into synaptosomes of rat brain. J. Pharmacol. Exp. Ther. 193, 804–811.
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume49, Issue3

September 1987

Pages 821-827

Lack of Selectivity Between the Uptake of [³H] Adrenaline and [³H]Noradrenaline into Rat Hypothalamic Slices

Abstract

Abbreviations used:

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Lack of Selectivity Between the Uptake of [3H] Adrenaline and [3H]Noradrenaline into Rat Hypothalamic Slices

Abstract

Abbreviations used:

References

Citing Literature

References

Related

Information

Lack of Selectivity Between the Uptake of [³H] Adrenaline and [³H]Noradrenaline into Rat Hypothalamic Slices