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Distribution of Components of the SNARE Complex in Relation to Transmitter Release Sites at the Frog Neuromuscular Junction

Jeanne-Andrée Boudier

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

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Nathalie Charvin,

Nathalie Charvin

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

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Jean-Louis Boudier,

Corresponding Author

Jean-Louis Boudier

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

Correspondence to: J.-A. Boudier, as aboveSearch for more papers by this author

Mohammed Fathallah,

Mohammed Fathallah

Centre d'Immunologie INSERM-CNRS, Parc Scientifique de Luminy, case 906, 13288 Marseille Cedex 09, France

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Mitsuo Tagaya,

Mitsuo Tagaya

Tokyo University of Pharmacy and Life Science, Hachiojii, 192–03 Tokyo, Japan

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Masami Takahashi,

Masami Takahashi

Mitsubishi Kasei Institute of Life Science, Machida, 194 Tokyo, Japan

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Michael J. Seagar,

Michael J. Seagar

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

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Jeanne-Andrée Boudier,

Jeanne-Andrée Boudier

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

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Nathalie Charvin,

Nathalie Charvin

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

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Jean-Louis Boudier,

Corresponding Author

Jean-Louis Boudier

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

Correspondence to: J.-A. Boudier, as aboveSearch for more papers by this author

Mohammed Fathallah,

Mohammed Fathallah

Centre d'Immunologie INSERM-CNRS, Parc Scientifique de Luminy, case 906, 13288 Marseille Cedex 09, France

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Mitsuo Tagaya,

Mitsuo Tagaya

Tokyo University of Pharmacy and Life Science, Hachiojii, 192–03 Tokyo, Japan

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Masami Takahashi,

Masami Takahashi

Mitsubishi Kasei Institute of Life Science, Machida, 194 Tokyo, Japan

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Michael J. Seagar,

Michael J. Seagar

INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Bd. Pierre Dramard, 13916 Marseille Cedex 20, France

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First published: March 1996

https://doi.org/10.1111/j.1460-9568.1996.tb01239.x

Citations: 28

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Abstract

At the frog neuromuscular junction, neurotransmitter release sites are regularly spaced at 1 μm intervals along the nerve terminal, directly facing postsynaptic folds which contain a high density of acetylcholine receptors. Immunostaining and laser confocal scanning microscopy were used to compare the distribution of presynaptic proteins implicated in exocytosis with that of fluorescent α-bungarotoxin. Syntaxin, synaptosome-associated 25 kDa protein and calcium channels were located predominantly at release sites. Synaptobrevin (vesicle-associated membrane protein) was distributed in the cytoplasm of the nerve terminal, presumably in the packets of microvesicles associated with each active zone. N-Ethylmaleimide-sensitive fusion protein (NSF) and soluble NSF attachment proteins (αβSNAP) displayed a diffuse distribution throughout the terminal cytoplasm and also colocalized in distinct concentrated zones adjacent to the presynaptic membrane.

Abbreviations:

αβSNAP: soluble NSF attachment proteins
AChR: acetylcholine receptors
Cy5: cyanine 5
FITC: fluorescein isothiocyanate
HEPES: N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulphonic acid)
NSF: N-ethylmaleimide-sensitive fusion protein
R-αβBuTx: rhodamine-tagged bungarotoxin
SNAP-25: synaptosome-associated 25 kDa protein
SNARE: SNAP receptor complex
VAMP: vesicle-associated membrane protein
ωGVIA: ω-conotoxin GVIA

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Volume8, Issue3

March 1996

Pages 545-552

Distribution of Components of the SNARE Complex in Relation to Transmitter Release Sites at the Frog Neuromuscular Junction

Abstract

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Distribution of Components of the SNARE Complex in Relation to Transmitter Release Sites at the Frog Neuromuscular Junction

Abstract

Abbreviations:

References

Citing Literature

References

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