ANTIMICROBIAL ACTIVITY OF CEFAZOLIN-IMPREGNATED MESH GRAFTS

Corresponding Author

Dilek Kilic

* Departments of Infectious Disease and Clinical Microbiology and ‡ Surgery, Kirikkale University, Kirikkale , and † Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey

Associate Professor Dilek Kilic, Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Kirikkale University, Kirikkale 71100, Turkey.
Email: [email protected]Search for more papers by this author

Canan Agalar,

Canan Agalar

Search for more papers by this author

Eylem Ozturk,

Eylem Ozturk

Search for more papers by this author

Emir B. Denkbas,

Emir B. Denkbas

Search for more papers by this author

Abdullah Cime,

Abdullah Cime

Search for more papers by this author

Fatih Agalar,

Fatih Agalar

Search for more papers by this author

Dilek Kilic,

Corresponding Author

Dilek Kilic

Canan Agalar,

Canan Agalar

Search for more papers by this author

Eylem Ozturk,

Eylem Ozturk

Search for more papers by this author

Emir B. Denkbas,

Emir B. Denkbas

Search for more papers by this author

Abdullah Cime,

Abdullah Cime

Search for more papers by this author

Fatih Agalar,

Fatih Agalar

Search for more papers by this author

First published: 27 March 2007

https://doi.org/10.1111/j.1445-2197.2007.04029.x

Citations: 15

D. Kilic MD; C. Agalar MD; E. Ozturk; E. B. Denkbas PhD; A. Cime MD; F. Agalar MD.

Share a link

Email
Wechat
Bluesky

Abstract

Background: The aim of this study is the preparation and characterization of cefazolin-impregnated meshes (Surgipro; Tyco Healthcare USSC, Norwalk, CT, USA) to be used as antimicrobial devices.

Methods: During the impregnation, poly(dl-lactide-co-glycolide) solution with cephazolin in dichloromethane was used as coating material. In vitro release experiment was carried out first; later cefazolin-impregnated meshes were evaluated for the characteristics of antimicrobial efficacy and in the last part of the study native and cefazolin-impregnated meshes were implanted in the rats. Cefazolin content was proposed as the effective parameter to control the cefazolin release rate and it was concluded that the higher amounts of initial cefazolin content caused higher release rates. In all cases (or with different cefazolin content for each mesh), the release rates were very rapid in the first 24 h and in the following periods rather slow release rates were obtained.

Results: Antimicrobial activity was increased in the case of cefazolin-impregnated form and this efficiency was also increased by the higher amount of cefazolin in certain mesh pieces. Similar antimicrobial activities were observed in the in vitro studies.

Conclusion: In this study, almost all of the cefazolin-impregnated mesh grafts showed very high antimicrobial activity compared with the bare mesh (or mesh without cefazolin).

References

1 Junge K, Rosch R, Klinge U et al. Gentamicin supplementation of polyvinylidenfluoride mesh materials for infection prophylaxis. Biomaterials 2005; 26: 787–93.
10.1016/j.biomaterials.2004.02.070
CAS PubMed Web of Science® Google Scholar
2 Junge K, Klinge U, Klosterhalfen B, Rosch R, Stumpf M, Schumpelick V. Foreign body reaction to meshes used for the repair of abdominal wall hernias. Eur. J. Surg. 1999; 165: 665–73.
10.1080/11024159950189726
PubMed Web of Science® Google Scholar
3 Gaonkar TA, Sampath LA, Modak SM. Evaluation of the antimicrobial efficacy of urinary catheters impregnated with antiseptics in an in vitro urinary tract model. Infect. Control Hosp. Epidemiol. 2003; 24: 506–13.
10.1086/502241
PubMed Web of Science® Google Scholar
4 Alexander JW, Kaplan JZ, Altemeier WA. Role of suture materials in the development of wound infection. Ann. Surg. 1967; 165: 192–9.
10.1097/00000658-196702000-00005
CAS PubMed Web of Science® Google Scholar
5 Giacometti A, Cirioni O, Ghiselli R et al. Efficacy of quinupristin–dalfopristin in preventing vascular graft infection due to Staphylococcus epidermidis with intermediate resistance to glycopeptides. Antimicrob. Agents Chemother. 2002; 46: 2885–8.
10.1128/AAC.46.9.2885-2888.2002
CAS PubMed Web of Science® Google Scholar
6 Dunne WM Jr, Mason EO Jr, Kaplan SL. Diffusion of rifampin and vancomycin through a Staphylococcus epidermidis biofilm. Antimicrob. Agents Chemother. 1993; 37: 2522–6.
10.1128/AAC.37.12.2522
CAS PubMed Web of Science® Google Scholar
7 Troy MG, Dong QS, Dobrin PB, Hecht D. Do topical antibiotics provide improved prophylaxis against bacterial growth in the presence of polypropylene mesh? Am. J. Surg. 1996; 171: 391–3.
10.1016/S0002-9610(97)89616-X
CAS PubMed Web of Science® Google Scholar
8 Darouiche RO, Fowler VG Jr, Adal K, Kielhofner M, Mansouri D, Reller LB. Antimicrobial activity of prosthetic heart valve sewing cuffs coated with minocycline and rifampin. Antimicrob. Agents Chemother. 2002; 46: 543–5.
10.1128/AAC.46.2.543-545.2002
CAS PubMed Web of Science® Google Scholar
9 Citak MS, Cue JI, Peyton JC, Malangoni MA. The critical relationship of antibiotic dose and bacterial contamination in experimental infection. J. Surg. Res. 1992; 52: 127–30.
10.1016/0022-4804(92)90292-8
CAS PubMed Web of Science® Google Scholar
10 Darouiche RO, Raad II, Heard SO et al. A comparison of two antimicrobial-impregnated central venous catheters. Catheter Study Group. N. Engl. J. Med. 1999; 340: 1–8.
10.1056/NEJM199901073400101
CAS PubMed Web of Science® Google Scholar
11 Bandyk DF. Antibiotics – why so many and when should we use them? Semin. Vasc. Surg. 2002; 15: 268–74.
10.1016/S0895-7967(02)70026-4
PubMed Google Scholar
12 Kim CY, Kumar A, Sampath L, Sokol K, Modak S. Evaluation of an antimicrobial-impregnated continuous ambulatory peritoneal dialysis catheter for infection control in rats. Am. J. Kidney Dis. 2002; 39: 165–73.
10.1053/ajkd.2002.29911
PubMed Web of Science® Google Scholar
13 Liedberg H, Lundeberg T. Silver alloy coated catheters reduce catheter-associated bacteriuria. Br. J. Urol. 1990; 65: 379–81.
10.1111/j.1464-410X.1990.tb14760.x
CAS PubMed Web of Science® Google Scholar
14 Webster J, Hood RH, Burridge CA, Doidge ML, Phillips KM, George N. Water or antiseptic for periurethral cleaning before urinary catheterization: a randomized controlled trial. Am. J. Infect. Control 2001; 29: 389–94.
10.1067/mic.2001.117447
CAS PubMed Web of Science® Google Scholar
15 National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, 5th edn. Wayne, PA: National Committee for Clinical Laboratory Standards; Approved standard M7–A5, 2004.
Web of Science® Google Scholar
16 Henke PK, Bergamini TM, Rose SM, Richardson JD. Current options in prosthetic vascular graft infection. Am. Surg. 1998; 64: 39–45.
CAS PubMed Web of Science® Google Scholar
17 Woodford N, Johnson AP, Morrison D, Speller DC. Current perspectives on glycopeptide resistance. Clin. Microbiol. Rev. 1995; 8: 585–615.
10.1128/CMR.8.4.585
CAS PubMed Web of Science® Google Scholar
18 McManus AT, Goodwin CW, Pruitt BA Jr. Observations on the risk of resistance with the extended use of vancomycin. Arch. Surg. 1998; 133: 1207–11.
10.1001/archsurg.133.11.1207
CAS PubMed Web of Science® Google Scholar
19 Raad I, Hachem R, Tcholakian RK, Sherertz R. Efficacy of minocycline and EDTA lock solution in preventing catheter-related bacteremia, septic phlebitis, and endocarditis in rabbits. Antimicrob. Agents Chemother. 2002; 46: 327–32.
10.1128/AAC.46.2.327-332.2002
CAS PubMed Web of Science® Google Scholar
20 Obst G, Gagnon RF, Haris J, Prentis J, Richards GK. The activity of rifampin and analogs against Staphylococcus epidermidis biofilms in ACAPD environment model. Am. J. Nephrol. 1989; 9: 414–20.
10.1159/000168004
CAS PubMed Web of Science® Google Scholar
21 Van Wijngaerden E, Peetermans WE, Vandersmissen J, Van Lierde S, Bobbaers H, Van Eldere J. Foreign body infection: a new rat model for prophylaxis and treatment. J. Antimicrob. Chemother. 1999; 44: 669–74.
10.1093/jac/44.5.669
CAS PubMed Web of Science® Google Scholar
22 Agalar C, Eroglu E, Sari M, Sari A, Daphan C, Agalar F. The effect of G-CSF in an experimental MRSA graft infection in mice. J. Invest. Surg. 2005; 18: 227–31.
10.1080/08941930500248581
PubMed Web of Science® Google Scholar
23 Classen DC, Evans RS, Pestotnik SL, Horn SD, Menlove RL, Burke JP. The timing of prophylactic administration of antibiotics and the risk of surgical-wound infection. N. Engl. J. Med. 1992; 326: 281–6.
10.1056/NEJM199201303260501
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume77, Issue4

April 2007

Pages 256-260

ANTIMICROBIAL ACTIVITY OF CEFAZOLIN-IMPREGNATED MESH GRAFTS

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

ANTIMICROBIAL ACTIVITY OF CEFAZOLIN-IMPREGNATED MESH GRAFTS

Abstract

References

Citing Literature

References

Related

Information