Journal of Gastroenterology and Hepatology

Nuclear factor-kappa B as a promising target for selenium chemoprevention in rat hepatocarcinogenesis

Corresponding Author

Nasar Y Alwahaibi

Department of Biomedical Sciences, Faculty of Allied Health Sciences, National University of Malaysia, Julan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia; and

Dr Nasar Y Alwahaibi, Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia (UKM), Julan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Email: [email protected]Search for more papers by this author

Siti B Budin,

Siti B Budin

Department of Biomedical Sciences, Faculty of Allied Health Sciences, National University of Malaysia, Julan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia; and

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Jamaludin Mohamed,

Jamaludin Mohamed

Department of Biomedical Sciences, Faculty of Allied Health Sciences, National University of Malaysia, Julan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia; and

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Aisha Alhamdani,

Aisha Alhamdani

Aisha Alhamdani, Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman

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Nasar Y Alwahaibi,

Corresponding Author

Nasar Y Alwahaibi

Department of Biomedical Sciences, Faculty of Allied Health Sciences, National University of Malaysia, Julan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia; and

Siti B Budin,

Siti B Budin

Department of Biomedical Sciences, Faculty of Allied Health Sciences, National University of Malaysia, Julan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia; and

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Jamaludin Mohamed,

Jamaludin Mohamed

Department of Biomedical Sciences, Faculty of Allied Health Sciences, National University of Malaysia, Julan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia; and

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Aisha Alhamdani,

Aisha Alhamdani

Aisha Alhamdani, Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman

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First published: 25 March 2010

https://doi.org/10.1111/j.1440-1746.2009.06160.x

Citations: 9

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Abstract

Background and Aims: Selenium's molecular mechanism for cancer chemoprevention remains unknown. We aimed to study the gene expression of nuclear factor-κB (NF-κB), tumor growth factor-α (TGF-α) and cyclin D1 and the effects of sodium selenite using preventive and therapeutic approaches in chemically-induced hepatocarcinogenesis in rats.

Methods: Rats were divided randomly into six groups: negative control, positive control (diethyl nitrosamine [DEN] + 2-acetylaminofluorene [2-AAF]), preventive group, preventive control (respective control for preventive group), therapeutic group and therapeutic control (respective control for therapeutic group). The relative gene expression of NF-κB, TGF-α and cyclin D1 in liver tissues were measured using real-time polymerase chain reaction.

Results: The findings showed that the gene expression of NF-κB in the preventive group and its respective control was significantly lower (P < 0.05) when compared with both the negative and positive controls. However, the expression of NF-κB in the positive controls and therapeutic group was significantly higher (P < 0.05) when compared with the negative controls. The expression of TGF-α and cyclin D1 was insignificant in all groups.

Conclusion: The inhibition of the NF-κB pathway in the initiation phase of hepatocarcinogenesis could be a promising target for selenium chemoprevention. However, further studies are required.

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Volume25, Issue4

April 2010

Pages 786-791

Nuclear factor-kappa B as a promising target for selenium chemoprevention in rat hepatocarcinogenesis

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Nuclear factor-kappa B as a promising target for selenium chemoprevention in rat hepatocarcinogenesis

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