ORIGINAL ARTICLE

Reduced expression of myocardin and serum response factor in the cavernous tissue of diabetic rats

S.-H. He

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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A.-Y. Wei,

A.-Y. Wei

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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Y. Yang,

Y. Yang

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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Y.-W. Hu,

Y.-W. Hu

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China

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X.-G. Luo,

X.-G. Luo

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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Y. Liu,

Y. Liu

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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T. Zhang,

T. Zhang

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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S.-H. He,

S.-H. He

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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A.-Y. Wei,

A.-Y. Wei

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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Y. Yang,

Y. Yang

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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Y.-W. Hu,

Y.-W. Hu

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China

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X.-G. Luo,

X.-G. Luo

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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Y. Liu,

Y. Liu

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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T. Zhang,

T. Zhang

Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, China

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First published: 26 September 2011

https://doi.org/10.1111/j.1439-0272.2011.01218.x

Citations: 4

An-Yang Wei, PhD, Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
Tel.: +86 20 6164 2231;
Fax: +86 20 6164 2231;
E-mail: [email protected]

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Summary

This study aimed to investigate the expression of myocardin and serum response factor (SRF) in the cavernous tissue of diabetic rats. The experimental diabetes model was induced in 8-week-old male Sprague-Dawley rats (200–220 g) by a single administration of streptozotocin. Both the diabetes mellitus group (DM group, n = 20) and the control group (NDM group, n = 10) were injected with a low dose of apomorphine to allow for the measurement and comparison of the corresponding penile erections. Western blot and qRT-PCR were used to determine the protein and mRNA expression levels of myocardin and SRF. Erectile function was significantly decreased in the DM group compared with the control group (P < 0.001). The mRNA and protein expression levels of myocardin and SRF were reduced in the cavernous tissue of diabetic rats compared with the control group (P < 0.001). It is concluded that diabetes inhibits the mRNA and protein expression of both myocardin and SRF in the cavernous tissue. This could play a key role in the development of erectile dysfunction in diabetic rats.

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Reduced expression of myocardin and serum response factor in the cavernous tissue of diabetic rats

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Reduced expression of myocardin and serum response factor in the cavernous tissue of diabetic rats

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