Acid-Base Regulation by Azolla spp. with N2 as Sole N Source and with Supplementation by NH+4 or NO−3
Corresponding Author
J. A. Raven
Department of Biological Sciences, University of Dundee, U.K.
Institut für Botanik und Mikrobiologie der Technischen Universität München Lehrstuhl für Botanik Arcisstraße 21 D-8000 München 2 Federal Republic of Germany
Department of Biological Sciences University of Dundee Dundee DD1 4HN United Kingdom
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C. Rothemund
Present Address: Institut für Botanik und Mikrobiologie der Technischen Universität München, Lehrstuhl für Botanik, Arcisstrasse 21; D-8000 München 2, FRG
Institut für Botanik und Mikrobiologie der Technischen Universität München Lehrstuhl für Botanik Arcisstraße 21 D-8000 München 2 Federal Republic of Germany
Department of Biological Sciences University of Dundee Dundee DD1 4HN United Kingdom
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B. Wollenweber
Department of Biological Sciences, University of Dundee, U.K.
Institut für Botanik und Mikrobiologie der Technischen Universität München Lehrstuhl für Botanik Arcisstraße 21 D-8000 München 2 Federal Republic of Germany
Department of Biological Sciences University of Dundee Dundee DD1 4HN United Kingdom
Search for more papers by this authorCorresponding Author
J. A. Raven
Department of Biological Sciences, University of Dundee, U.K.
Institut für Botanik und Mikrobiologie der Technischen Universität München Lehrstuhl für Botanik Arcisstraße 21 D-8000 München 2 Federal Republic of Germany
Department of Biological Sciences University of Dundee Dundee DD1 4HN United Kingdom
Search for more papers by this authorCorresponding Author
C. Rothemund
Present Address: Institut für Botanik und Mikrobiologie der Technischen Universität München, Lehrstuhl für Botanik, Arcisstrasse 21; D-8000 München 2, FRG
Institut für Botanik und Mikrobiologie der Technischen Universität München Lehrstuhl für Botanik Arcisstraße 21 D-8000 München 2 Federal Republic of Germany
Department of Biological Sciences University of Dundee Dundee DD1 4HN United Kingdom
Search for more papers by this authorCorresponding Author
B. Wollenweber
Department of Biological Sciences, University of Dundee, U.K.
Institut für Botanik und Mikrobiologie der Technischen Universität München Lehrstuhl für Botanik Arcisstraße 21 D-8000 München 2 Federal Republic of Germany
Department of Biological Sciences University of Dundee Dundee DD1 4HN United Kingdom
Search for more papers by this authorAbstract
Diazotrophic cultures of three species of Azolla (Az. caroliniana, Az. microphylla, Az. pinnata) symbiotic with Anabaena azollae accumulated some 0.10–0.24 mol organic anion per mol N assimilated (0.010–0.018 mol organic anion per mol C assimilated), with a corresponding efflux of 0.05–0.11 mol H+ per mol N assimilated (0.006–0.009 mol H+ per mol C assimilated). These values are lower than those found for terrestrial diazotrophic vascular plants; this may be related to the decreased possibility of increasing Fe and P availability by rhizosphere acidification in a free-floating plant. Modification of the organic anion content, and of the quantity (and direction) of H+ exchange with the medium, with 5 mol m−3 NH+4 or NO−3 added to diazotrophic cultures, are consistent with substantial N acquisition from combined N as well as N2 assimilation. This conclusion is consistent with previously published work with 15N.
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