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Quantitative Analysis of Lea and Leb Antigens in Human Saliva
Baojie Wang,
Kazuko Akiyama,
Hiroshi Kimura MD, PhD,
Baojie Wang
Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
Search for more papers by this authorKazuko Akiyama
Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
Search for more papers by this authorCorresponding Author
Hiroshi Kimura MD, PhD
Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
Department of Legal Medicine Kurume University School of Medicine Kurume, Fukuoka 830, JapanSearch for more papers by this authorBaojie Wang,
Kazuko Akiyama,
Hiroshi Kimura MD, PhD,
Baojie Wang
Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
Search for more papers by this authorKazuko Akiyama
Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
Search for more papers by this authorCorresponding Author
Hiroshi Kimura MD, PhD
Department of Legal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
Department of Legal Medicine Kurume University School of Medicine Kurume, Fukuoka 830, JapanSearch for more papers by this authorAbstract
We have measured the H type 1, Lea and Leb antigens in the saliva from 129 Japanese individuals by a time-resolved europium ion fluorometric immunoassay using artificial antigen-albumin complexes as the reference substances. We confirmed that the amount of Leb was larger than that of Lea in the saliva from secretors (Lea–b+) and vice versa in the saliva from nonsecretors (Lea+b–). Unexpectedly, we discovered appreciable amounts of Leb with small amounts of H type 1 in the saliva from the nonsecretors. The concentration of Leb was about 10, 6 and 35% of the concentration of the Lea in the saliva from the nonsecretors of the A, B and O groups, respectively. The possible formation of Leb from Lea, in addition to the formation of Leb from H type 1, in the salivary glands is discussed.
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