Immunochemical Characterization of a Monoclonal Anti-Leb Blood Grouping Reagent
Corresponding Author
L. Messeter
Blood Bank, University Hospital, Lund, Sweden
Blood Bank, University Hospital, Lund (Sweden)Search for more papers by this authorT. Brodin
Department of Tumor Immunology, Wallenberg Laboratory, Lund, Sweden
Search for more papers by this authorK.-A. Karlsson
Department of Medical Biochemistry, University of Gothenburg, Sweden
Search for more papers by this authorD. Zopf
National Cancer Institute, The National Institutes of Health, Bethesda, Md., USA
Search for more papers by this authorCorresponding Author
L. Messeter
Blood Bank, University Hospital, Lund, Sweden
Blood Bank, University Hospital, Lund (Sweden)Search for more papers by this authorT. Brodin
Department of Tumor Immunology, Wallenberg Laboratory, Lund, Sweden
Search for more papers by this authorK.-A. Karlsson
Department of Medical Biochemistry, University of Gothenburg, Sweden
Search for more papers by this authorD. Zopf
National Cancer Institute, The National Institutes of Health, Bethesda, Md., USA
Search for more papers by this authorAbstract
A haemagglutinating monoclonal anti-LebL antibody has been obtained from a mouse-mouse hybridoma after immunization with the Leb-active oligosaccharide lacto-N-difucohexaose I, coupled to edestin. Of 13 other antibodies obtained, one was able to agglutinate enzyme-treated erythrocytes of all ABO groups and three more were considered to be anti-LebH haemagglutinins. The remaining nine antibodies did not agglutinate red cells. The specificity of the stronger of the anti-LebL haemagglutinins was examined by studying its binding to a number of glycoconjugates and oligosaccharides.
References
- 1 Race, R.R.; Sanger, R.: Blood groups in man; 6th ed., p. 339 (Blackwell, Oxford 1975).
- 2 Marcus, D.M.; Bastani, A.M.; Rosenfield, R.E.; Grollman, A.P.: Studies of blood group substances. II. Haemagglutinating properties of caprine antisera to human Lea and Leb blood group substances. Transfusion 7: 227–280 (1967).
- 3 Zopf, D.A.; Ginsburg, A.; Ginsburg, V.: Goat antibody directed against a human blood group hapten, lacto-N-difucohexaose I. J. Immun. 115: 1525–1529 (1975).
- 4 Brockhaus, M.; Magnani, J.L.; Blaszczyk, M.; Steplewski, S.; Koprowski, H.; Karlsson, K.-A.; Larson, G.; Ginsburg, V.: Monoclonal antibodies directed against the human Leb blood group antigen. J. biol. Chem. 256: 13223–13225 (1981).
- 5 Zopf, D.A.; Tsai, C.M.; Ginsburg, V.: Carbohydrate antigens: coupling of oligosaccharide-phenethylamine derivatives to edestin by diazotization and characterization of antibody specificity by radioimmunoassay. Meth. Enzym. 50: 163–169 (1978).
- 6 Smith, D.F.; Zopf, D.A.; Ginsburg, V.: Carbohydrate antigens. Coupling of oligosaccharide phenethylamine-isothiocyanate derivatives to bovine serum albumin. Meth. Enzym. 50: 169–171 (1978).
- 7 Ellens, D.J.; Gielkens, A.L.J.: A simple method for the purification of 5-aminosalicylate. Application of the product as substrate in ELISA. J. immunol. Methods 37: 325–332 (1980).
- 8
Kennett, R.H.: Monoclonal antibodies, pp. 365–367 (Plenum Press, New York 1980).
10.1007/978-1-4615-7505-4 Google Scholar
- 9 Nowinski, R.C.; Lostrom, M.E.; Tam, M.R.; Stone, M.R.; Burnette, W.M.: The isolation of hybrid cell lines producing monoclonal antibodies against the p15 (E) protein of ecotropic murine leukemia. Virology 93: 111–126 (1979).
- 10 Lundblad, A.: Oligosaccharides from human urine. Meth. Enzym. 50: 226–235 (1978).
- 11 Strecker, G.; Trentesaux-Chauvet, C.; Riazi-Farzad, T.; Fournet, B.; Bouquelet, S.; Montreuil, J.: Mise en évidence d'une oligosaccharidosurie associée à diverses mélituries et détermination de la structure des oligosaccharides excrétés. Hypothèse concernant l'origine des oligosaccharides des liquides biologiques. C.r. hebd. Séanc. Acad. Sci., Paris 277: 1569–1572 (1973).
- 12 Kobata, A.; Ginsburg, V.: Oligosaccharides of human milk, IV. Isolation and characterization of a new hexasaccharide, lacto-N-neohexaose. Archs Biochem. Biophys. 150: 273–281 (1972).
- 13 Smith, E.L.; McKibbin, J.M.; Karlsson, K.-A.; Pascher, I.; Samuelsson, B.E.; Li, Y.-T.; Li, S.-C.: Characterization of a human intestinal fucolipid with blood group Lea activity. J. biol. Chem. 250: 6059–6064 (1975).
- 14 Falk, K.-E.; Karlsson, K.-A.; Samuelsson, B.E.: Proton nuclear magnetic resonance analysis of anomeric structure of glycosphingolipids. Lewis-active and Lewis-like substances. Archs Biochem. Biophys. 192: 191–202 (1979).
- 15 McKibbin, J.M.; Spencer, W.A.; Smith, E.L.; Månsson, J.-E.; Karlsson, K.-A.; Samuelsson, B.E.; Li, Y.-T.; Li, S.-C.: Lewis blood group fucolipids and their isomers from human and canine intestine. J. biol. Chem. 257: 755–760 (1982).
- 16 Seaman, M.J.; Chalmers, D.G.; Franks, D.: Siedler: an antibody which reacts with A1 Le(a–b +) red cells. Vox Sang. 15: 25–30 (1968).
- 17 Engvall, E.; Perlmann, P.: Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J. Immun. 109: 129–135 (1972).
- 18 Löw, B.: A practical method using papain and incomplete Rh-antibodies in routine Rh grouping. Vox Sang. 5: 94–98 (1955).
- 19 Ouchterlony, Ö.: Diffusion-in-gel methods for immunological analysis. Prog. Allergy, vol. 5, pp. 1–78 (Karger, Basel 1958).
- 20 Hakomori, S.I.; Strycharz, G.D.: Investigations on cellular blood group substances. I. Isolation and chemical composition of blood group ABH and Leb isoantigens of sphingoglycolipid nature. Biochemistry 7: 1279–1286 (1968).
- 21 Marcus, D.M.; Cass, L.E.: Glycosphingolipids with Lewis blood group activity: uptake by human erythrocytes. Science 164: 553–555 (1969).
- 22 Lemieux, R.U.: Human blood groups and carbohydrate chemistry. Chem. Soc. Rev. 7: 423–452 (1978).
Citing Literature
