ISOLATION AND CHARACTERIZATION OF HIGHLY PURIFIED ALPHA-1-ANTITRYPSIN
Marie-Therese Plancot
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorAndre Delacourte
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorCorresponding Author
Dr. Kia-Ki Han
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Laboratoire des Protéines Fibreuses Unité nd̀ 16 de l'INSERM Place de Verdun 59045 Lille Cédex FranceSearch for more papers by this authorMichel Dautrevaux
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorGerard Biserte
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorMarie-Therese Plancot
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorAndre Delacourte
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorCorresponding Author
Dr. Kia-Ki Han
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Laboratoire des Protéines Fibreuses Unité nd̀ 16 de l'INSERM Place de Verdun 59045 Lille Cédex FranceSearch for more papers by this authorMichel Dautrevaux
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorGerard Biserte
Laboratoire des Protéines Fibreuses, Unité nd̀16 de l'INSERM, Lille Cédex, France
Search for more papers by this authorAbstract
Highly purified human alpha-1-antitrypsin (phenotype MM) was obtained by an original method of preparative electrophoresis. The criteria of homogeneity were assured by one arc in crossed immunoelectrophoresis and one band on polyacrylamide gel. A unique N-terminal amino acid (pyroglutamic acid) and a unique C-terminal residue (lysine) were identified. Determined by gel electrophoresis, its molecular weight was 47,000 daltons.
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