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The chromosomal integration site for the Streptomyces plasmid SLP1 is a functional tRNATyr gene essential for cell viability
Martin Vögtli,
Stanley N. Cohen,
Martin Vögtli
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305. USA.
Institut für Zellbiologie, ETH Zürich, Honggerberg, CH-8093 Zürich, Switzerland.
Search for more papers by this authorCorresponding Author
Stanley N. Cohen
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305. USA.
*Tel. (415) 723 5315; Fax (415)725 1536.Search for more papers by this authorMartin Vögtli,
Stanley N. Cohen,
Martin Vögtli
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305. USA.
Institut für Zellbiologie, ETH Zürich, Honggerberg, CH-8093 Zürich, Switzerland.
Search for more papers by this authorCorresponding Author
Stanley N. Cohen
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305. USA.
*Tel. (415) 723 5315; Fax (415)725 1536.Search for more papers by this authorSummary
The genetic element SLP1 exists in nature as a single DNA segment integrated into the genome of Streptomyces coelicolor. Upon mating with Streptomyces lividans, a closely related species, SLP1 undergoes precise excision from its chromosomal site and is transferred into the recipient where it integrates chromosomally. Previous work has shown that integration and excision involve site-specific recombination between a chromosomal site, attB, and a virtually identical sequence, attP, on SLP1. We demonstrate here by means of gene replacement that a tRNATvr sequence that overlaps part of the attB site of S. lividans is both biologically functional and essential for cell viability. The requirement for this tRNA gene has been used to stabilize the inheritance of a segrationally unstable plasmid in cells lacking a chromosomal attB site. The evolution of an essential DNA locus as an attachment site for a chromosomally integrating genetic element represents a novel mechanism of biological adaptation.
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