Escherichia coli up-regulates proinflammatory cytokine expression in granulocyte/macrophage lineages of CD34+ stem cells via p50 homodimeric NF-κB
Corresponding Author
J. M. KIM
Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine, Seoul,
Jung Mogg Kim MD, Department of Microbiology, Hanyang University College of Medicine, 17 Haengdang-dong, Sungdong-gu, Seoul 133–791, Korea. E-mail: [email protected]Search for more papers by this authorY.-K. OH
Department of Microbiology, Pochon CHA University College of Medicine, Kyunggi-do,
Search for more papers by this authorY.-J. KIM
Department of Science, Joongbu University, Choongnam,
Search for more papers by this authorJ. YOUN
Department of Anatomy and Cell Biology, Hanyang University College of Medicine, Seoul, and
Search for more papers by this authorM.-J. AHN
Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea
Search for more papers by this authorCorresponding Author
J. M. KIM
Department of Microbiology and Institute of Biomedical Science, Hanyang University College of Medicine, Seoul,
Jung Mogg Kim MD, Department of Microbiology, Hanyang University College of Medicine, 17 Haengdang-dong, Sungdong-gu, Seoul 133–791, Korea. E-mail: [email protected]Search for more papers by this authorY.-K. OH
Department of Microbiology, Pochon CHA University College of Medicine, Kyunggi-do,
Search for more papers by this authorY.-J. KIM
Department of Science, Joongbu University, Choongnam,
Search for more papers by this authorJ. YOUN
Department of Anatomy and Cell Biology, Hanyang University College of Medicine, Seoul, and
Search for more papers by this authorM.-J. AHN
Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea
Search for more papers by this authorSUMMARY
Umbilical cord blood has emerged as an alternative source of haematopoietic CD34+ cells for allogeneic stem cell transplantation. Although bacteraemia induced by Escherichia coli is considered one of the complications of transplantation, expression of proinflammatory cytokines is poorly understood. In this study, we report the altered expression of proinflammatory cytokines in CD34+ cells and their in vitro cultured cells following E. coli infection. CD34+ stem cells and their cultured cells up-regulated expression of proinflammatory cytokines such as interleukin (IL)-1α, IL-6, IL-8 and tumour necrosis factor (TNF)-α after infection with E. coli. Expression of the proinflammatory cytokines was generated mainly by the granulocyte-macrophage lineages. E. coli infection activated the signals of p50/p50 nuclear factor-kappaB (NF-κB) homodimers and IκB kinase. Furthermore, inhibition of NF-κB activation lowered the up-regulated expression of the proinflammatory cytokines. These results suggest that CD34+ cells and their cultured cells infected with E. coli induce the expression of proinflammatory cytokines via the NF-κB pathway.
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