An experimental influenza subunit vaccine (iscom): induction of protective immunity to challenge infection in mice after intranasal or subcutaneous administration
Corresponding Author
K. LÖVGREN
The National Veterinary Institute, Department of Virology, Uppsala, Sweden
K. Lövgren, The National Veterinary Institute, Department of Virology, Box 585, BMC, S-751 23 Uppsala, Sweden.Search for more papers by this authorH. KÅBERG
The National Veterinary Institute, Department of Virology, Uppsala, Sweden
Search for more papers by this authorB. MOREIN
The National Veterinary Institute, Department of Virology, Uppsala, Sweden
Swedish University of Agricultural Sciences, College of Veterinary Microbiology, Section of Virology, Uppsala, Sweden
Search for more papers by this authorCorresponding Author
K. LÖVGREN
The National Veterinary Institute, Department of Virology, Uppsala, Sweden
K. Lövgren, The National Veterinary Institute, Department of Virology, Box 585, BMC, S-751 23 Uppsala, Sweden.Search for more papers by this authorH. KÅBERG
The National Veterinary Institute, Department of Virology, Uppsala, Sweden
Search for more papers by this authorB. MOREIN
The National Veterinary Institute, Department of Virology, Uppsala, Sweden
Swedish University of Agricultural Sciences, College of Veterinary Microbiology, Section of Virology, Uppsala, Sweden
Search for more papers by this authorSUMMARY
An experimental influenza virus (A/PR/8/34(H1N1)) vaccine was tested and evaluated in mice. The mice were inoculated once or twice intranasally or subcutancously with 1 or 10 μg of iscoins prior to challenge with high doses of live virus. It was demonstrated that two intranasal administrations were as efficient as two s.c. administrations, both routes inducing high levels of antibody and protection against challenge infection. With a one-dose regimen, the s.c. route induced a somewhat higher antibody response than the intranasal route; this might be explained by technical difficulties connected with an intranasal administration.
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