AICDA expression in BCR/ABL1-positive acute lymphoblastic leukaemia is associated with a peculiar gene expression profile
Monica Messina
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Equal contribution.
Search for more papers by this authorSabina Chiaretti
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Equal contribution.
Search for more papers by this authorIlaria Iacobucci
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorSimona Tavolaro
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorAnnalisa Lonetti
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorSimona Santangelo
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorLoredana Elia
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorCristina Papayannidis
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorAntonella Vitale
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorAnna Guarini
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorGiovanni Martinelli
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorRobin Foà
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorMonica Messina
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Equal contribution.
Search for more papers by this authorSabina Chiaretti
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Equal contribution.
Search for more papers by this authorIlaria Iacobucci
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorSimona Tavolaro
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorAnnalisa Lonetti
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorSimona Santangelo
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorLoredana Elia
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorCristina Papayannidis
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorAntonella Vitale
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorAnna Guarini
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorGiovanni Martinelli
Department of Haematology/Oncology “L. and A. Seràgnoli” S. Orsola Malpighi Hospital, University of Bologna, Bologna
Search for more papers by this authorRobin Foà
Division of Haematology, Department of Cellular Biotechnologies and Haematology, “Sapienza” University of Rome, Rome
Search for more papers by this authorSummary
Activation-induced cytidine deaminase (AICDA) initiates somatic hypermutation and class-switch recombination of immunoglobulin (Ig) genes and induces mutations also in non-Ig genes. AICDA aberrant expression was detected in B-lineage acute lymphoblastic leukaemia (B-ALL), particularly BCR/ABL1+ B-ALL; patients expressing AICDA carried more copy number alterations than ‘AICDA-negative’ cases. To determine the role of AICDA, AICDA expression and gene expression profiling were studied in adult BCR/ABL1+ B-ALL. Patients displaying the full-length isoform AICDA are characterized by up-regulation of DNA repair/replication and cell cycle genes, suggesting their involvement in the genetic instability of BCR/ABL1+ B-ALL.
Supporting Information
Fig S1. Unsupervised analysis results. Unsupervised hierarchical clustering based on the expression of 757 filtered genes. Each row represents a probeset, each column a sample. Color scale: blue and red indicate the lowest and highest expression levels, respectively.
Fig S2. Q-PCR results. Low ΔCT values correspond to high gene expression levels. Boxplots define the median values, 25–75% of values around the median, and the range of values. Six specific genes of ‘AICDA-FL’ samples resulting from anova.
Table SI. Genes and primers for Q-PCR validation. Correlation coefficient between oligonucleotide microarrays and Q-PCR results is also given.
Table SII. Genes over-expressed in ‘AICDA-FL’ patients selected by anova belonging to DNA Repair/Replication, Regulation of Cell Cycle, Nucleotide Metabolism categories.
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| BJH_8449_sm_figS1-2-tableS1-2.pdf298.2 KB | Supporting info item |
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