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An in vivo experimental model for effects of topical retinoic acid in human skin

Corresponding Author

C.E.M. GRIFFITHS

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

Dr C. K. M. Griffiths, Department of Dermatology, University of Michigan Medical Center, 1910 Taubman Center, Ann Arbor, MI 48109-0314, U. S. ASearch for more papers by this author

L.J. FINKEL,

L.J. FINKEL

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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M.G. TKANFAGLIA,

M.G. TKANFAGLIA

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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T.A. HAMILTON,

T.A. HAMILTON

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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J.J. VOORHEES,

J.J. VOORHEES

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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C.E.M. GRIFFITHS,

Corresponding Author

C.E.M. GRIFFITHS

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

Dr C. K. M. Griffiths, Department of Dermatology, University of Michigan Medical Center, 1910 Taubman Center, Ann Arbor, MI 48109-0314, U. S. ASearch for more papers by this author

L.J. FINKEL,

L.J. FINKEL

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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M.G. TKANFAGLIA,

M.G. TKANFAGLIA

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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T.A. HAMILTON,

T.A. HAMILTON

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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J.J. VOORHEES,

J.J. VOORHEES

Dermatopharmacology Unit, Department of Dermatology, University of Michigan Medical Center, Ann Arbor, Michiganm U. S. A.

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First published: October 1993

https://doi.org/10.1111/j.1365-2133.1993.tb03163.x

Citations: 70

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Summary

An occlusive patch-test assay has been developed for assessment of topical retinoid action in human epidermis. Previous work with this assay has demonstrated marked epidermal hyperplasia in skin treated with topical all-trans-retinoic acid for 4 days and similar effects with the local irritant, sodium lauryl sulphate. To investigate the capabilities of this assay further, a time-course and dose-response were performed with all-trans-retinoic acid, and a comparison made with sodium lauryl sulphate.

At no time, between 1 and 4 days, could the clinical or histological effects of 0.1% and 0.025% cream formulations of all-trans-retinoic acid be distinguished from each other. Epidermal hyperplasia was used to generate a 4-day dose-response for all-trans-retinoic acid at concentrations from 0. 00 l to 0. 025% dissolved in a 70% ethanol/30% propylene glycol vehicle. All-trans-retinoic acid could he successfully differentiated from sodium lauryl sulphate at 2 days by virtue of its greater ability to increase epidermal thickness, spongiosis and glycosaminoglycan deposition.

It appears that although all-trans-retinoic acid and sodium lauryl sulphate produce similar epidermal histological changes at 4 days, significant differences at earlier time-points suggest differing mechanisms of action. In addition, this in vivo human assay is able to provide potency ranking for doses of all-trans-retinoic acid, and may predict clinical efficacy of retinoids in improvement of acne and/or photodamage.

References

1 Kligman LH, Kligman AM. The effect on rhino mouse skin of agents which influence keratinization and exfoliation. J Invest Dermatol 1979; 73: 354–8.
10.1111/1523-1747.ep12550409
CAS PubMed Web of Science® Google Scholar
2 Ashton RE, Connor MJ, Lowe NJ. Histologic changes in the skin of rhino mouse (hr hr) Induced by retinoids. J Invest Dermatol 1984; 82: 632–5.
10.1111/1523-1747.ep12261472
PubMed Web of Science® Google Scholar
3 Connor MJ. Ashton RE. Lowe NJ. A comparative study of the induction of epidermal hyperplasia by natural and synthetic retinoids. J Pharmacol Exp Ther 1986; 237: 31–5.
CAS PubMed Web of Science® Google Scholar
4 Kligman LH. Effects of all-trans-retinoic acid on the dermis of hairiess mice. J Am Acad Dermatol 1986; 15: 779–85.
10.1016/S0190-9622(86)70234-X
PubMed Web of Science® Google Scholar
5 Boucher M, Chatelus A, Ferracin J et al. Quantification of epidermal histological changes induced by topical retinoids and CD271 in the rhino mouse model using a standardized image analysis technique. Skin Pharmacol 1991; 4: 65–73.
10.1159/000210926
PubMed Web of Science® Google Scholar
6 Varani J, Gendimenico GJ, Shah B et al. A direct comparison of pharmacolgic effects of retinoids on skin cells in vitro in vitro Skin Pharmacol 1991; 4: 254–61.
CAS PubMed Web of Science® Google Scholar
7 Griffiths CEM, Rosenthal DS, Reddy AP et al. Short-term retinoic acid treatment increases in vivo, but decreases in vitro. epidermal transglutaminase-K enzyme activity and immunoreactivity, J Invest Dermatol 1992; 99: 283–8.
10.1111/1523-1747.ep12616626
CAS PubMed Web of Science® Google Scholar
8 Fisher GJ, Esmann J, Griffiths CEM et al. Cellular, immunologic and biochemical characterization of topical retinoic acid-treated human skin, J Invest Dermatol 1991; 96: 699–707.
10.1111/1523-1747.ep12470632
CAS PubMed Web of Science® Google Scholar
9 Weiss JS, Ellis CN, Headington JT et al. Topical tretinoin improves photoaged skin. A double-blind, vehicle-controiled study. JAMA 1988; 259: 527–32.
10.1001/jama.1988.03720040019020
CAS PubMed Web of Science® Google Scholar
10 Bhawan J, Gonzalez-Serva A, Nehal K et al. Effects of tretinoin on photodamaged skin. A histologic study, Arch Dermatol 1991; 127: 666–72.
10.1001/archderm.1991.01680040074006
CAS PubMed Web of Science® Google Scholar
11 Rafal ES, Griffiths CEM, Ditre CM et al. Topical tretinoin (retinoic acid) treatment for liver spots associated with photodamag N Engl J Med 1992; 326: 368–74.
10.1056/NEJM199202063260603
PubMed Web of Science® Google Scholar
12 Kligman AM, Grove GL, Hirose R, Leyden JJ. Topical tretinoin for photoaged skin. J Am Acad Dermatol 1990; 15: 836–59.
10.1016/S0190-9622(86)70242-9
Web of Science® Google Scholar
13 Lever L, Kumar P, Marks R. Topical retinoic acid for the treatment of solar damage, Br J Dermatol 1990; 122: 91–8.
10.1111/j.1365-2133.1990.tb08244.x
CAS PubMed Web of Science® Google Scholar
14 Caputo R, Monti M, Motta S et al. The treatment of visible signs of senescence: the Italian experience. Br J Dermatol 1990; 122 (Suppl. 35): 97–103.
10.1111/j.1365-2133.1990.tb16133.x
PubMed Web of Science® Google Scholar
15 Weinstein GD, Nigra TP, Pochi PE et al. Topical tretinoin for treatment of photodamaged skin. A multicenter study. Arch Dermatol 1991; 127: 659–65.
10.1001/archderm.1991.01680040067005
CAS PubMed Web of Science® Google Scholar
16 Olsen EA, Katz I, Levine N et al. Tretinoin emollient cream: a new therapy for photodamaged skin. J Am Acad Dermatol 1992; 26: 215–24.
10.1016/0190-9622(92)70030-J
CAS PubMed Web of Science® Google Scholar
17 Fisher GJ, Tavakkol A, Griffiths CEM et al. Differential modulation of transforming growth factor-beta 1 expression and mucin deposition by retinoic acid and sodium lauryl sulfate in human skin, J Invest Dermatol 1992; 98: 102–8.
10.1111/1523-1747.ep12495896
CAS PubMed Web of Science® Google Scholar
18 Petkovich M, Brand NJ, Krust A, Chambon P. A human retinoic acid receptor which belongs to the family of nuclear receptors. Nature 1987; 330: 444–50.
10.1038/330444a0
CAS PubMed Web of Science® Google Scholar
19 Brand N, Petkovich M, Krust A, et al. Identification of a second human retinoic acid receptor. Nature 1988; 332: 850–3.
10.1038/332850a0
CAS PubMed Web of Science® Google Scholar
20 Krust A, Kastner P, Petkovieh M et al. A third human retinoic acid receptor hRAR-γ. Proc Natl Acad Sci USA 1989; 86: 5310–14.
10.1073/pnas.86.14.5310
CAS PubMed Web of Science® Google Scholar
21 Elder JT, Fisher GJ, Zhang QY et al. Retinoic acid receptor gene expression in human skin, J Invest Dermatol 1991; 96: 425–33.
10.1111/1523-1747.ep12469889
CAS PubMed Web of Science® Google Scholar
22 Rees JL, Redfern CPF. Expression of the α and β retinoic acid receptors in skin. J Invest Dermatol 1989; 92: 818–20.
10.1111/1523-1747.ep12284435
Web of Science® Google Scholar
23 Astrom A, Tavakkol A, Pettersson U et al. Molecular cloning of two human cellular retinoic acid-binding proteins (CRABP), J Biol Chem 1991; 266: 7662–6.
Google Scholar
24 Elder JT, Cromie MA, Griffiths CEM et al. Stimulus-selective induction of CRABP-II mRNA: a marker for retinoic acid action in human skin, J Invest Dermatol 1993; 100: 356–9.
10.1111/1523-1747.ep12471816
CAS Google Scholar
25 Varani J, Astrom A, Griffiths CEM, Voorhees JJ. Induction of proliferation of growth-inhibited keratinocytes and fibroblasts in monolayer culture by sodium Iauryl sulfate: comparison with all-trans retinoic acid, J Invest Dermatol 1991; 97: 917–21.
10.1111/1523-1747.ep12491682
CAS PubMed Web of Science® Google Scholar

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Volume129, Issue4

October 1993

Pages 389-394

An in vivo experimental model for effects of topical retinoic acid in human skin

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An in vivo experimental model for effects of topical retinoic acid in human skin

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