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Identification of Enterohemorrhagic Escherichia coli O157-Specific DNA Sequence Obtained from Amplified Fragment Length Polymorphism Analysis
Akihiko Tokunaga,
Masanori Kawano,
Masatoshi Okura,
Sunao Iyoda,
Haruo Watanabe,
Ro Osawa,
Akihiko Tokunaga
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorMasanori Kawano
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorMasatoshi Okura
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorSunao Iyoda
Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, 162-8640 Japan
Search for more papers by this authorHaruo Watanabe
Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, 162-8640 Japan
Search for more papers by this authorCorresponding Author
Ro Osawa
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Address correspondence to Dr. Ro Osawa, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, Hyogo 657-8501, Japan. Fax: +81-78-803-5804. E-mail: [email protected]Search for more papers by this authorAkihiko Tokunaga,
Masanori Kawano,
Masatoshi Okura,
Sunao Iyoda,
Haruo Watanabe,
Ro Osawa,
Akihiko Tokunaga
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorMasanori Kawano
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorMasatoshi Okura
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorSunao Iyoda
Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, 162-8640 Japan
Search for more papers by this authorHaruo Watanabe
Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, 162-8640 Japan
Search for more papers by this authorCorresponding Author
Ro Osawa
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, 657-8501 Japan
Address correspondence to Dr. Ro Osawa, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, Hyogo 657-8501, Japan. Fax: +81-78-803-5804. E-mail: [email protected]Search for more papers by this authorAbstract
An approximately 1.1 kbp fragment that was commonly observed only in the enterohemorrhagic Escherichia coli (EHEC) O157 strains in an analysis of amplified fragment length polymorphism was found to be a partial gene sequence encoding the locus of toxB and a useful molecular marker for the identification of EHEC O157.
Abbreviations
-
- A/E
-
- attaching and effacing
-
- AFLP
-
- amplified fragment length polymorphism
-
- DNA
-
- deoxyribonucleic acid
-
- EHEC
-
- enterohemorrhagic Escherichia coli
-
- EPEC
-
- enteropathogenic Escherichia coli
-
- STEC
-
- Shiga-toxin-producing Escherichia coli
-
- Stx
-
- Shiga toxin
-
- PCR
-
- polymerase chain reaction.
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