Original Article

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Characterization of the Cyclophilin of Trichophyton mentagrophytes

Corresponding Author

Rui Kano

Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, 252-8510 Japan

Address correspondence to Dr. Rui Kano, Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, Kanagawa, 252-8510, Japan, Fax + 81-466-84-3649, E-mail: [email protected]Search for more papers by this author

Yuka Nakamura,

Yuka Nakamura

Department of Dermatology, Teikyo University School of Medicine, Itabashi-ku, Tokyo, 173-8605 Japan

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Shinichi Watanabe,

Shinichi Watanabe

Department of Dermatology, Teikyo University School of Medicine, Itabashi-ku, Tokyo, 173-8605 Japan

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Hajime Tsujimoto,

Hajime Tsujimoto

Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyuo-ku, Tokyo, 113-8657 Japan

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Atsuhiko Hasegawa,

Atsuhiko Hasegawa

Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, 252-8510 Japan

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Rui Kano,

Corresponding Author

Rui Kano

Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, 252-8510 Japan

Yuka Nakamura,

Yuka Nakamura

Department of Dermatology, Teikyo University School of Medicine, Itabashi-ku, Tokyo, 173-8605 Japan

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Shinichi Watanabe,

Shinichi Watanabe

Department of Dermatology, Teikyo University School of Medicine, Itabashi-ku, Tokyo, 173-8605 Japan

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Hajime Tsujimoto,

Hajime Tsujimoto

Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyuo-ku, Tokyo, 113-8657 Japan

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Atsuhiko Hasegawa,

Atsuhiko Hasegawa

Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, 252-8510 Japan

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First published: 14 November 2013

https://doi.org/10.1111/j.1348-0421.2000.tb01245.x

Citations: 1

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Abstract

A genetic approach to cyclophilins in a dermatophyte, Trichophyton mentagrophytes, was carried out. The nucleotide and deduced amino acid sequences of the cyclophilin of T. mentagrophytes shared about 70% sequence similarity with those of Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans. However, the first 21 amino acid and the C-terminal amino acid regions of 188 to 226 of the T. mentagrophytes cyclophilin were distinct from those of the other fungal cyclophilins. The recombinant glutathione 5-transferase (GST)-T. mentagrophytes cyclophilin fusion protein produced by Escherichia coli was purified. The protease digest of the fusion protein had a molecular weight of about 13 kDa and peptidyl-prolyl cis-trans isomerase (PPI) activity. This digest protein from T. mentagrophytes was confirmed to be cyclophilin by proving PPI activity.

Abbreviations

cDNA: complementary DNA
CsA: cyclosporin A
DNA: deoxyribonucleic acid
DTM: dermatophyte test medium
EDTA: ethylenediamine-N, N, N′, N′-tetra acetic acid disodium salt dihydrate
GST: glutathione 5-transferase
IPTG: isopropyl β-D-thiogalactoside
PCR: polymerase chain reaction
PPI: peptidyl-prolyl cis-trans isomerase
SDS: sodium dodecyl sulfate
TE buffer: 10 mM Tris-HCl, pH 8.0 and 1 mM EDTA

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Volume44, Issue1

January 2000

Pages 51-56

Characterization of the Cyclophilin of Trichophyton mentagrophytes

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Characterization of the Cyclophilin of Trichophyton mentagrophytes

Abstract

Abbreviations

REFERENCES

Citing Literature

References

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