Comparison of Three Electron Microscopy Techniques for the Detection of Human Rotaviruses
Pierre Trépanier
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorRobert Alain
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorValeriu Micusan
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorBernadette McLaughlin
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorLaurent Berthiaume
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorPierre Trépanier
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorRobert Alain
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorValeriu Micusan
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorBernadette McLaughlin
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorLaurent Berthiaume
Université du Québec, Centre de Recherche en Virologie, and Centre de Recherche en Immunologie, Institut Armand-Frappier, C.P. 100, Laval, Québec, Canada, H7N 4Z3
Laboratoire de Microbiologie, Hôpital Ste-Justine, Montréal, Canada
Search for more papers by this authorAbstract
Rotavirus detection by direct electron microscopy was compared with direct and indirect immune electron microscopy techniques. The latter two approaches permitted the enumeration of 25 and 103 times more rotaviruses respectively, than direct electron microscopy. Also, 70% and 90% of the virus particles were aggregated by direct and indirect immune electron microscopy techniques respectively, thus facilitating their detection.
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