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VARIATION OF ENZYME ACTIVITIES AT A BRANCHED PATHWAY INVOLVED IN THE UTILIZATION OF GLUCONATE IN ESCHERICHIA COLI
Ing-Nang Wang,
Daniel E. Dykhuizen,
Ing-Nang Wang
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843 E-mail: [email protected]
Search for more papers by this authorDaniel E. Dykhuizen
Department of Ecology and Evolution, State University of New York at Stony Brook, Stony Brook, New York 11794–5245 E-mail: [email protected]
Search for more papers by this authorIng-Nang Wang,
Daniel E. Dykhuizen,
Ing-Nang Wang
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843 E-mail: [email protected]
Search for more papers by this authorDaniel E. Dykhuizen
Department of Ecology and Evolution, State University of New York at Stony Brook, Stony Brook, New York 11794–5245 E-mail: [email protected]
Search for more papers by this authorAbstract
Abstract.— Twenty-four strains of Escherichia coli from the ECOR collection were characterized for growth rate in gluconate minimal salts medium and for Vmax and Km of the three enzymes (gluconokinase, 6-phosphogluconate dehydrogenase, and 6-phosphogluconate dehydratase) that form a branch point for the utilization of gluconate. A total of 11 characters–growth rate, three Vmax values, four Km values, and three Vmax/Km values–were determined for these 24 ECOR strains. Most of the characters were normally distributed. Statistical tests showed that growth rate is significantly less variable than enzyme activities. Also, analyses of variance showed significant differences among strains and among the extant five genetic groups of E. coli for the characters measured. A Mantel test showed that, for some characters, closely related strains shared similar character values. Two hypotheses regarding the relationships between growth rate and enzyme activity and between various enzyme activities were tested. None of the expected correlations between growth rate and enzyme activity or between enzyme activities was detected. The results were discussed in terms of metabolic control analysis and neutral theory.
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