miR-24-3p obstructs the proliferation and migration of human skin fibroblasts after thermal injury by targeting PPAR-β and positively regulated by NF-κB
Xu Cui
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorXu Huang
Department of Hyperbaric Oxygen, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorMitao Huang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorSituo Zhou
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorGuo Le
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorWenchang Yu
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorMengting Duan
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorBimei Jiang
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, China
Search for more papers by this authorJizhang Zeng
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorJie Zhou
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorXiaoyuan Huang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorPengfei Liang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Pihong Zhang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Correspondence
Pihong Zhang, Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Kaifu District, Changsha, Hunan 410008, China.
Email: [email protected]
Search for more papers by this authorXu Cui
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorXu Huang
Department of Hyperbaric Oxygen, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorMitao Huang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorSituo Zhou
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorGuo Le
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorWenchang Yu
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorMengting Duan
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorBimei Jiang
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, China
Search for more papers by this authorJizhang Zeng
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorJie Zhou
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorXiaoyuan Huang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorPengfei Liang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Pihong Zhang
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, China
Correspondence
Pihong Zhang, Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Kaifu District, Changsha, Hunan 410008, China.
Email: [email protected]
Search for more papers by this authorFunding information
This work was supported by the National Natural Science Foundation of China (No. 81974287, 81971820, 81770306) and the Key R&D Project in Hunan Province (No. 2018SK2089)
Abstract
Thermal injury repair is a complex process during which the maintenance of the proliferation and migration of human skin fibroblasts (HSFs) exert a crucial role. MicroRNAs have been proven to exert an essential function in repairing skin burns. This study delves into the regulatory effects of miR-24-3p on the migration and proliferation of HSFs that have sustained a thermal injury, thereby, providing deeper insight into thermal injury repair pathogenesis. The PPAR-β protein expression level progressively increased in a time-dependent manner on the 12th, 24th and 48th hour following the thermal injury of the HSFs. The knockdown of PPAR-β markedly suppressed the proliferation of and migration of HSF. Following thermal injury, the knockdown also promoted the inflammatory cytokine IL-6, TNF-α, PTGS-2 and P65 expression. PPAR-β contrastingly exhibited an opposite trend. A targeted relationship between PPAR-β and miR-24-3p was predicted and verified. miR-24-3p inhibited thermal injured HSF proliferation and migration and facilitated inflammatory cytokine expression through the regulation of PPAR-β. p65 directly targeted the transcriptional precursor of miR-24 and promoted miR-24 expression. A negative correlation between miR-24-3p expression level and PPAR-β expression level in rats’ burnt dermal tissues was observed. Our findings reveal that miR-24-3p is conducive to rehabilitating the denatured dermis, which may be beneficial in providing effective therapy of skin burns.
CONFLICT OF INTEREST
The authors confirm that there are no conflicts of interest.
Open Research
DATA AVAILABILITY STATEMENT
The authors confirm that the data supporting the findings of this study are available within the article.
Supporting Information
| Filename | Description |
|---|---|
| exd14517-sup-0001-FigureS1.epsimage/postscript, 1.9 MB | Figure S1. PPAR-β inhibits the NF-kB pathway and inflammatory cytokine in non-thermally injured HSFs. (A) The effect of PPAR-β on p65 and p-p65 protein in non-thermally injured HSFs was determined using Western blot assay. (B) The effect of PPAR-β on the expression of inflammatory cytokine IL-6, TNF-α, and PTGS2 in non-thermally injured HSFs was determined using PCR assay. N=3; **p < 0.01 compared to si-NC group; #p < 0.05, ##p < 0.01 compared to Vector group. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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