Mannan-binding lectin promotes keratinocyte to produce CXCL1 and enhances neutrophil infiltration at the early stages of psoriasis
Jiaqi Zeng
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorXi Chen
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorKe Lei
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorDi Wang
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorLin Lin
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorYajie Wang
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorYao Li
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorYunzhi Liu
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorLiyun Zhang
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorCorresponding Author
Daming Zuo
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Guangdong Provincial Key Laboratory of Proteomics, Southern Medical University, Guangzhou, China
Correspondence
Ledong Sun, Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, 510900, China.
Email: [email protected]
Daming Zuo, School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, 510515, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Ledong Sun
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Correspondence
Ledong Sun, Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, 510900, China.
Email: [email protected]
Daming Zuo, School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, 510515, China.
Email: [email protected]
Search for more papers by this authorJiaqi Zeng
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorXi Chen
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorKe Lei
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorDi Wang
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorLin Lin
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorYajie Wang
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorYao Li
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorYunzhi Liu
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorLiyun Zhang
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Search for more papers by this authorCorresponding Author
Daming Zuo
School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, China
School of Basic Medical Sciences, Department of Immunology, Southern Medical University, Guangzhou, China
Guangdong Provincial Key Laboratory of Proteomics, Southern Medical University, Guangzhou, China
Correspondence
Ledong Sun, Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, 510900, China.
Email: [email protected]
Daming Zuo, School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, 510515, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Ledong Sun
Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China
Correspondence
Ledong Sun, Department of Dermatology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, 510900, China.
Email: [email protected]
Daming Zuo, School of Laboratory Medicine and Biotechnology, Institute of Molecular Immunology, Southern Medical University, Guangzhou, 510515, China.
Email: [email protected]
Search for more papers by this authorFunding information
This work was supported in part by National Natural Science Foundation of China 81671568 and Guangdong Natural Science Foundation 2017A030313542.
Abstract
Psoriasis is a chronic, relapsing inflammatory skin disorder. Numerous experimental evidence and therapeutic evidence have shown that the innate immune response is critical for the pathogenesis and development of psoriasis. Mannan-binding lectin (MBL), a prototypic pattern recognition molecule of the innate immune system, plays an essential role in the host defense against certain infections and also appears to be a major regulator of inflammation. In this study, we investigated the function of MBL on the course of experimental murine imiquimod (IMQ)-induced psoriasis. Our data showed that MBL-deficient (MBL−/−) mice exhibited attenuated skin damage characterized by greatly decreased erythema compared with wild-type control mice during the early stages of IMQ-induced psoriasis-like skin inflammation. The reduced skin inflammation in MBL−/− mice was associated with the decreased infiltration of neutrophils. Furthermore, we have determined that MBL deficiency limited the chemokine CXCL1 production from skin keratinocytes upon IMQ stimulation, which might be responsible for the impaired skin recruitment of neutrophils. Additionally, we have provided the data that MBL protein promotes the IMQ-induced expression of CXCL1 and activation of MAPK/NF-κB signalling pathway in human keratinocyte HaCaT cells in vitro. In summary, our study revealed an unexpected role of MBL on keratinocyte function in skin, thus offering a new insight into the pathogenic mechanisms of psoriasis.
CONFLICT OF INTEREST
The authors have no conflict of interest to declare.
Supporting Information
| Filename | Description |
|---|---|
| exd13995-sup-0001-FigS1.docxWord document, 302.1 KB | Fig S1. MBL deficiency does not affect the frequency of IL-17- producing cells in skin draining lymph nodes from IMQ-treated mice. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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