European Journal of Clinical Investigation

Volume 45, Issue 1 pp. 70-80

Original Article

Development of a dietary-induced metabolic syndrome model using miniature pigs involvement of AMPK and SIRT1

Sin-Jin Li,

Sin-Jin Li

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Chia-Hsin Liu,

Chia-Hsin Liu

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Chei-Wei Chang,

Chei-Wei Chang

Taitung Animal Propagation Station, Livestock Research Institute Council of Agriculture, Taitung County, Taiwan

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Hsien-Pin Chu,

Hsien-Pin Chu

Taitung Animal Propagation Station, Livestock Research Institute Council of Agriculture, Taitung County, Taiwan

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Kuen-Jaw Chen,

Kuen-Jaw Chen

Taitung Animal Propagation Station, Livestock Research Institute Council of Agriculture, Taitung County, Taiwan

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Harry J. Mersmann,

Harry J. Mersmann

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Shih-Torng Ding,

Shih-Torng Ding

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

Institute of Biotechnology, National Taiwan University, Taipei, Taiwan

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Chun-Han Chu,

Chun-Han Chu

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Ching-Yi Chen,

Corresponding Author

Ching-Yi Chen

orcid.org/0000-0002-5776-084X

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

Correspondence to: Ching-Yi Chen, Department of Animal Science and Technology, National Taiwan University, Present address: 50, Lane 155, Sec 3, Keelung Rd, Taipei 106, Taiwan. Tel.: +886-2-33665283; fax: +886-2-27324070; e-mail: [email protected]Search for more papers by this author

Sin-Jin Li,

Sin-Jin Li

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Chia-Hsin Liu,

Chia-Hsin Liu

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Chei-Wei Chang,

Chei-Wei Chang

Taitung Animal Propagation Station, Livestock Research Institute Council of Agriculture, Taitung County, Taiwan

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Hsien-Pin Chu,

Hsien-Pin Chu

Taitung Animal Propagation Station, Livestock Research Institute Council of Agriculture, Taitung County, Taiwan

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Kuen-Jaw Chen,

Kuen-Jaw Chen

Taitung Animal Propagation Station, Livestock Research Institute Council of Agriculture, Taitung County, Taiwan

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Harry J. Mersmann,

Harry J. Mersmann

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Shih-Torng Ding,

Shih-Torng Ding

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

Institute of Biotechnology, National Taiwan University, Taipei, Taiwan

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Chun-Han Chu,

Chun-Han Chu

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

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Ching-Yi Chen,

Corresponding Author

Ching-Yi Chen

orcid.org/0000-0002-5776-084X

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

First published: 06 November 2014

https://doi.org/10.1111/eci.12370

Citations: 14

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Abstract

Background

During the progression of the metabolic syndrome (MetS), cardiovascular diseases (CVD) appear clinically in many individuals and cause death. As a result, it is essential to set up an optimal animal model to study the mechanism of MetS leading to CVD. SIRT1 and AMPK are the master regulators of lipid and carbohydrate metabolism. The objective of this study was to establish a miniature pig model of Western diet-induced MetS and investigate the role of SIRT1/AMPK during MetS development.

Materials and Methods

Five-month-old Lee-Sung (LS) and Lanyu (LY) minipigs were each randomly assigned to two groups: control diet (C) and Western diet (W), in a 6-month experimental period.

Results

Western diet caused obesity in both minipig models. Compared with the CLS pigs, WLS pigs exhibited hypercholesterolaemia. However, WLY pigs maintained a similar plasma lipid profile to the CLY pigs. Western diet caused a lower antioxidant capacity in the liver of both pig models. WLS pigs had higher triglyceride accumulation in the liver than CLS pigs, whereas WLY and CLY pigs had similar hepatic triglyceride accumulation. Compared with CLS pigs, WLS pigs had a lower hepatic SIRT1 expression, whereas WLY pigs had a higher expression of AMPK, FOXO1 and SIRT1 than CLY pigs.

Conclusion

Long-term feeding of the Western diet to Lee-Sung miniature pigs not only caused obesity but also induced MetS and fatty liver, whereas Western diet induced obesity in Lanyu pigs without metabolic dysfunctions. SIRT1/AMPK and their downstream pathways might be one of the possible regulators for pathological obesity in Lee-Sung pigs.

Supporting Information

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Volume45, Issue1

January 2015

Pages 70-80

Development of a dietary-induced metabolic syndrome model using miniature pigs involvement of AMPK and SIRT1

Abstract

Background

Materials and Methods

Results

Conclusion

Supporting Information

References

Citing Literature

References

Information

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Development of a dietary-induced metabolic syndrome model using miniature pigs involvement of AMPK and SIRT1

Abstract

Background

Materials and Methods

Results

Conclusion

Supporting Information

References

Citing Literature

References

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