Protein O-mannosyltransferase Rv1002c contributes to low cell permeability, biofilm formation in vitro, and mycobacterial survival in mice
Shufeng Yang
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
These authors have contributed equally to this work.
Search for more papers by this authorShaoguang Sui
Department of Emergency, The Second Hospital of Dalian Medical University, Dalian, China
These authors have contributed equally to this work.
Search for more papers by this authorYuanhua Qin
Department of Parasitology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Search for more papers by this authorHaibo Chen
Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China
Search for more papers by this authorShanshan Sha
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Search for more papers by this authorXin Liu
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Search for more papers by this authorCorresponding Author
Guoying Deng
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Guoying Deng, Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected] and Yufang Ma, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected]
Search for more papers by this authorCorresponding Author
Yufang Ma
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Guoying Deng, Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected] and Yufang Ma, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected]
Search for more papers by this authorShufeng Yang
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
These authors have contributed equally to this work.
Search for more papers by this authorShaoguang Sui
Department of Emergency, The Second Hospital of Dalian Medical University, Dalian, China
These authors have contributed equally to this work.
Search for more papers by this authorYuanhua Qin
Department of Parasitology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Search for more papers by this authorHaibo Chen
Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China
Search for more papers by this authorShanshan Sha
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Search for more papers by this authorXin Liu
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Search for more papers by this authorCorresponding Author
Guoying Deng
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Guoying Deng, Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected] and Yufang Ma, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected]
Search for more papers by this authorCorresponding Author
Yufang Ma
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China
Guoying Deng, Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected] and Yufang Ma, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China. e-mail: [email protected]
Search for more papers by this authorAbstract
Mycobacterium tuberculosis (M. tuberculosis) Rv1002c encodes the protein O-mannosyltransferase (PMT), which catalyzes the transfer of mannose to serine or threonine residues of proteins. We explored the function of PMT in vitro and in vivo. Rv1002c protein was heterogeneously overexpressed in nonpathogenic Mycobacterium smegmatis (named as MS_Rv1002c). A series of trials including mass spectrometry, transmission electron microscope, biofilm formation and antibiotics susceptibility were performed to explore the function of PMT on bacterial survival in vitro. Mouse experiments were carried out to evaluate the virulence of PMT in vivo. PMT decreased the cell envelope permeability and promoted microbial biofilm formation. PMT enhanced the mycobacterial survival in vivo and inhibited the release of pro-inflammatory cytokines in serum. The function might be associated with an increased abundance of some mannoproteins in culture filtrate (CF). PMT is likely to be involved in mycobacterial survival both in vivo and in vitro due to increasing the mannoproteins abundance in CF.
CONFLICT OF INTEREST
The authors declared no conflict of interest.
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