Enhanced Skin Regeneration by Herbal Extract-Coated Poly-L-Lactic Acid Nanofibrous Scaffold
Aytak Jouybar
Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
Search for more papers by this authorEhsan Seyedjafari
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Search for more papers by this authorCorresponding Author
Abdolreza Ardeshirylajimi
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Address correspondence and reprint requests to Abdolreza Ardeshirylajimi, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. E-mail: [email protected]; Iraj Pousti, Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran. E-mail: [email protected]Search for more papers by this authorAli Zandi-Karimi
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Search for more papers by this authorNikta Feizi
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Search for more papers by this authorMohammad-Mehdi Khani
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorCorresponding Author
Iraj Pousti
Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
Address correspondence and reprint requests to Abdolreza Ardeshirylajimi, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. E-mail: [email protected]; Iraj Pousti, Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran. E-mail: [email protected]Search for more papers by this authorAytak Jouybar
Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
Search for more papers by this authorEhsan Seyedjafari
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Search for more papers by this authorCorresponding Author
Abdolreza Ardeshirylajimi
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Address correspondence and reprint requests to Abdolreza Ardeshirylajimi, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. E-mail: [email protected]; Iraj Pousti, Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran. E-mail: [email protected]Search for more papers by this authorAli Zandi-Karimi
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Search for more papers by this authorNikta Feizi
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Search for more papers by this authorMohammad-Mehdi Khani
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Search for more papers by this authorCorresponding Author
Iraj Pousti
Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
Address correspondence and reprint requests to Abdolreza Ardeshirylajimi, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. E-mail: [email protected]; Iraj Pousti, Department of Anatomy, Science and Research Branch, Islamic Azad University, Tehran, Iran. E-mail: [email protected]Search for more papers by this authorAbstract
The wound healing process is directly related to the type of treatment. Existing methods of treatment are not responsive enough for severe wounds. The aim of this study was the potential capacity investigation of poly-L-lactic acid (PLLA) nanofibrous scaffolds coated by aloe vera gel for wound dressing applications. In this study, electrospinning method was used for preparing PLLA nanofibers, and after characterization by SEM and MTT, its influence on the wound healing process was investigated with and without aloe vera gel as a wound dressing in full-thickness skin defect in mice. Band-Aids were used as a positive control and vaseline gauze as a negative control. SEM and MTT assays confirmed the nanometer size and biocompatibility of fabricated nanofibers. Macroscopic and histopathological characteristics were evaluated at the end of days 7, 12, and 17 and their results showed that the gel-coated scaffold accelerated the wound-healing process compared with other groups. At the end of the experiment, it was shown that during the whole time of study, gel-coated scaffold had the highest overall repair score. Therefore, gel-coated PLLA scaffold would be an ideal construct for wound healing and skin regenerative medicine application.
Supporting Information
Additional Supporting Information may be found in the online version of this article at the publisher's web-site:
| Filename | Description |
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| aor12926-sup-0001-suppinfo1.tif9.2 MB |
Fig. S1. Macroscopic evaluation of wound healing in four study groups; with scaffold-gel and without scaffold-gel groups are treatment groups, Vaseline gauze and commercial sample groups are respectively negative control and positive control groups. (a) Scaffold with aloe vera gel, (b) scaffold without gel, (c) Vaseline gauze, (d) commercial sample on the 7th day after surgery, (e) scaffold with aloe vera gel, (f) scaffold without gel, (g) Vaseline gauze, (h) commercial sample on the 12th day after surgery, (i) scaffold with aloe vera gel, (j) scaffold without gel, (k) Vaseline gauze, (l) commercial sample on the 17th day after surgery. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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