The Anti-Inflammatory Impact of Omega-3 Polyunsaturated Fatty Acids During the Establishment of Endometriosis-Like Lesions
Jill A. Attaman
Department of Obstetrics and Gynecology, Harvard Vanguard Medical Associates, Boston, MA, USA
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
JA Attaman and AK Stanic contributed equally to this work.Search for more papers by this authorAleksandar K. Stanic
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
JA Attaman and AK Stanic contributed equally to this work.Search for more papers by this authorMinji Kim
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Search for more papers by this authorMaureen P. Lynch
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
Search for more papers by this authorBo R. Rueda
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
Search for more papers by this authorCorresponding Author
Aaron K. Styer
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
Correspondence
Aaron K. Styer, Vincent Center for Reproductive Biology, Thier 9, Massachusetts General Hospital, Boston, MA 02114, USA.
E-mail: [email protected]
Search for more papers by this authorJill A. Attaman
Department of Obstetrics and Gynecology, Harvard Vanguard Medical Associates, Boston, MA, USA
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
JA Attaman and AK Stanic contributed equally to this work.Search for more papers by this authorAleksandar K. Stanic
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
JA Attaman and AK Stanic contributed equally to this work.Search for more papers by this authorMinji Kim
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Search for more papers by this authorMaureen P. Lynch
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
Search for more papers by this authorBo R. Rueda
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
Search for more papers by this authorCorresponding Author
Aaron K. Styer
Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA
Vincent Department of Obstetrics and Gynecology, Vincent Center for Reproductive Biology, Massachusetts General Hospital, Boston, MA, USA
Correspondence
Aaron K. Styer, Vincent Center for Reproductive Biology, Thier 9, Massachusetts General Hospital, Boston, MA 02114, USA.
E-mail: [email protected]
Search for more papers by this authorAbstract
Problem
The anti-inflammatory impact of three polyunsaturated fatty acids (3-PUFA) in endometriosis is incompletely understood. The effect of 3-PUFA on endometriosis-like lesions is evaluated as a potential anti-inflammatory treatment target.
Method of Study
Wild Type (WT) and transgenic Fat-1 mice (high levels of endogenous 3-PUFA) were utilized in a uterine tissue transplant endometriosis model. Experimental donor × host pairs included: WT × WT (WW), WT × Fat-1 (WF), and Fat-1 × Fat-1 (FF). Cytokine content (IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-17A, IFN-γ, TNF-γ, MCP-1 and RANTES) and immunocellular composition in lesions was determined.
Results
Intralesion IL-6 in WF hosts was 99-fold lower than WW hosts (P = 0.03). Compared to WW host lesions, Cox-2 levels were decreased in WF [1.5-fold (P = 0.02)] and FF [1.2-fold (P = 0.01)] host lesions, respectively, and intralesion VEGF expression was increased [1.8-fold; P = 0.02 (WF) and 1.5-fold; P = 0.01 (FF)]. Lesions in FF hosts demonstrated reduced phosphohistone 3 expression (70%; P = 0.03) compared to WW control hosts.
Conclusions
Systemic host 3-PUFA levels influence immune, angiogenic, and proliferative factors implicated in the early establishment of endometriosis.
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